Importance of Propionibacterium acnes hemolytic activity in human intervertebral discs: A microbiological study.
Importance of Propionibacterium acnes hemolytic activity in human intervertebral discs: A microbiological study.
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DOI：
10.1371/journal.pone.0208144
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Slaby O
中科院分区：
文献类型：
--
作者：
Capoor MN;Ruzicka F;Sandhu G;Rollason J;Mavrommatis K;Ahmed FS;Schmitz JE;Raz A;Brüggemann H;Lambert PA;Fischetti VA;Slaby O
大多数慢性下背痛（CLBP）患者表现出退行性椎间盘疾病。在初始治疗性椎间盘切除术期间获得的椎间盘标本通常感染/定植有痤疮丙酸杆菌，痤疮丙酸杆菌是人类皮肤的革兰氏阳性菌。虽然与感染相关的疼痛通常归因于身体的炎症反应，但最近观察到革兰氏阳性细菌金黄色葡萄球菌通过分泌破坏神经元细胞膜的成孔α-溶血素直接激活伤害感受器。通过在羊血琼脂上孵育来评估在常规治疗性椎间盘切除术期间获得的培养椎间盘标本中痤疮丙酸杆菌的溶血活性。痤疮丙酸杆菌在羊血琼脂上显示的β-溶血模式是可变的，并且具有组依赖性。其溶血类型与其分子生物学类型相关。我们的研究结果提出了孔形成蛋白有助于慢性痤疮丙酸杆菌椎间盘感染和CLBP的发病机制和/或病理学的可能性，至少在一个子集的情况下。
Most patients with chronic lower back pain (CLBP) exhibit degenerative disc disease. Disc specimens obtained during initial therapeutic discectomies are often infected/colonized with Propionibacterium acnes, a Gram-positive commensal of the human skin. Although pain associated with infection is typically ascribed to the body’s inflammatory response, the Gram-positive bacterium Staphylococcus aureus was recently observed to directly activate nociceptors by secreting pore-forming α-hemolysins that disrupt neuronal cell membranes. The hemolytic activity of P. acnes in cultured disc specimens obtained during routine therapeutic discectomies was assessed through incubation on sheep-blood agar. The β-hemolysis pattern displayed by P. acnes on sheep-blood agar was variable and phylogroup-dependent. Their molecular phylogroups were correlated with their hemolytic patterns. Our findings raise the possibility that pore-forming proteins contribute to the pathogenesis and/or symptomology of chronic P. acnes disc infections and CLBP, at least in a subset of cases.
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影响因子：
--
作者：
Rollason J;McDowell A;Albert HB;Barnard E;Worthington T;Hilton AC;Vernallis A;Patrick S;Elliott T;Lambert P
通讯作者：
Lambert P
PGP 9.5阳性神经纤维的神经纤维神经模式。
影响因子：
2.4
作者：
Bailey JF;Liebenberg E;Degmetich S;Lotz JC
通讯作者：
Lotz JC
影响因子：
2.2
作者：
Soerensen, Meike;Mak, Tim N.;Brueggemann, Holger
通讯作者：
Brueggemann, Holger
影响因子：
168.9
作者：
Freemont, AJ;Peacock, TE;Jayson, MIV
通讯作者：
Jayson, MIV
影响因子：
3.7
作者：
Capoor MN;Ruzicka F;Schmitz JE;James GA;Machackova T;Jancalek R;Smrcka M;Lipina R;Ahmed FS;Alamin TF;Anand N;Baird JC;Bhatia N;Demir-Deviren S;Eastlack RK;Fisher S;Garfin SR;Gogia JS;Gokaslan ZL;Kuo CC;Lee YP;Mavrommatis K;Michu E;Noskova H;Raz A;Sana J;Shamie AN;Stewart PS;Stonemetz JL;Wang JC;Witham TF;Coscia MF;Birkenmaier C;Fischetti VA;Slaby O
通讯作者：
Slaby O