Genotypic and antimicrobial characterisation of Propionibacterium acnes isolates from surgically excised lumbar disc herniations.
Genotypic and antimicrobial characterisation of Propionibacterium acnes isolates from surgically excised lumbar disc herniations.
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DOI：
10.1155/2013/530382
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发表时间：
2013
影响因子：
--
通讯作者：
Lambert P
中科院分区：
文献类型：
--
作者：
Rollason J;McDowell A;Albert HB;Barnard E;Worthington T;Hilton AC;Vernallis A;Patrick S;Elliott T;Lambert P
厌氧皮肤痤疮丙酸杆菌是一个被低估的原因，人类感染和临床条件。以前的研究表明细菌在腰椎间盘突出和感染中的作用。为了进一步研究这一点，我们从64名腰椎间盘突出症患者中的每一位手术切除了5个活检样本。通过厌氧培养检测痤疮丙酸杆菌和其他细菌，然后进行生物化学和基于PCR的鉴定。总的来说，24/64例（38%）患者在切除的突出椎间盘组织中有痤疮丙酸杆菌的证据。使用recA和mAb分型方法，52%的分离株为II型（50%的培养阳性患者），而IA型菌株占分离株的28%（42%的患者）。III型（11%分离株; 21%患者）和IB型（9%分离株; 17%患者）检出频率较低。阿莫西林、环丙沙星、红霉素、利福平、四环素和万古霉素的MIC值最低（≤ 1 mg/L）。对夫西地酸的MIC为1-2 mg/L。甲氧苄啶和庆大霉素的MIC为2 ~ ≥4 mg/L。证明II型和III型菌株，这是不经常从皮肤中回收，占主导地位，在我们的分离收集（63%）表明，痤疮丙酸杆菌在腰椎间盘突出症的作用不应该轻易被驳回。
The anaerobic skin commensal Propionibacterium acnes is an underestimated cause of human infections and clinical conditions. Previous studies have suggested a role for the bacterium in lumbar disc herniation and infection. To further investigate this, five biopsy samples were surgically excised from each of 64 patients with lumbar disc herniation. P. acnes and other bacteria were detected by anaerobic culture, followed by biochemical and PCR-based identification. In total, 24/64 (38%) patients had evidence of P. acnes in their excised herniated disc tissue. Using recA and mAb typing methods, 52% of the isolates were type II (50% of culture-positive patients), while type IA strains accounted for 28% of isolates (42% patients). Type III (11% isolates; 21% patients) and type IB strains (9% isolates; 17% patients) were detected less frequently. The MIC values for all isolates were lowest for amoxicillin, ciprofloxacin, erythromycin, rifampicin, tetracycline, and vancomycin (≤1mg/L). The MIC for fusidic acid was 1-2 mg/L. The MIC for trimethoprim and gentamicin was 2 to ≥4 mg/L. The demonstration that type II and III strains, which are not frequently recovered from skin, predominated within our isolate collection (63%) suggests that the role of P. acnes in lumbar disc herniation should not be readily dismissed.
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影响因子：
11.8
作者：
Levy, Pierre Yves;Fenollar, Florence;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
3.7
作者：
Brzuszkiewicz E;Weiner J;Wollherr A;Thürmer A;Hüpeden J;Lomholt HB;Kilian M;Gottschalk G;Daniel R;Mollenkopf HJ;Meyer TF;Brüggemann H
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Brüggemann H
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4.7
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通讯作者：
Manniche, Claus
影响因子：
3.7
作者：
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Kilian, Mogens
影响因子：
1.9
作者：
Arnell, Kai;Cesarini, Kristina;Sjolin, Jan
通讯作者：
Sjolin, Jan