Inhibition of key digestive enzymes by cocoa extracts and procyanidins.
Inhibition of key digestive enzymes by cocoa extracts and procyanidins.
复制标题
DOI：
10.1021/jf200180n
复制
发表时间：
2011-05-25
影响因子：
6.1
通讯作者：
Lambert JD
中科院分区：
文献类型：
--
作者：
Gu Y;Hurst WJ;Stuart DA;Lambert JD
我们测定了可可提取物和原花青素对胰腺α-淀粉酶(PA)、胰腺脂肪酶(PL)和分泌型磷脂酶A2(PLA2)的体外抑制作用，并对其抑制动力学进行了研究。研究了Lavado、常规和荷兰加工的可可提取物以及可可原花青素(聚合度为2至10)。可可提取物和原花青素呈剂量依赖性抑制PA、PL和PLA2。Lavado可可提取物的抑制作用最强(IC_(50)=8.5~47μg/mL)。Log IC50与DP(R2>；0.93)呈负相关。动力学分析表明，普通可可提取物、五聚体和十聚体以混合方式抑制PL活性。五聚体和十聚体非竞争性抑制PLA2活性，而普通可可提取物竞争性抑制PLA2活性。我们的研究表明，可可多酚在体外可以抑制消化酶，并可能与低卡路里饮食一起在体重管理中发挥作用。
We determined the in vitro inhibitory effects of cocoa extracts and procyanidins against pancreatic α-amylase (PA), pancreatic lipase (PL) and secreted phospholipase A2 (PLA2), and characterized the kinetics of such inhibition. Lavado, regular and Dutch-processed cocoa extracts as well as cocoa procyanidins (degree of polymerization (DP) = 2 to 10) were examined. Cocoa extracts and procyanidins dose-dependently inhibited PA, PL and PLA2. Lavado cocoa extract was the most potent inhibitor (IC50 = 8.5 – 47 μg/mL). An inverse correlation between Log IC50 and DP (R2 > 0.93) was observed. Kinetic analysis suggested that regular cocoa extract, the pentamer and decamer inhibited PL activity in a mixed mode. The pentamer and decamer non-competitively inhibited PLA2 activity, whereas regular cocoa extract inhibited PLA2 competitively. Our study demonstrates that cocoa polyphenols can inhibit digestive enzymes in vitro, and may, in conjunction with a low calorie diet, play a role in body weight management.
登录
查看更多内容
影响因子：
120.7
作者：
Flegal, Katherine M.;Carroll, Margaret D.;Curtin, Lester R.
通讯作者：
Curtin, Lester R.
影响因子：
4.7
作者：
Richelle, M;Tavazzi, I;Offord, EA
通讯作者：
Offord, EA
影响因子：
5.6
作者：
Wang, Shu;Noh, Sang K.;Koo, Sung I.
通讯作者：
Koo, Sung I.
影响因子：
6.1
作者：
Miller, Kenneth B.;Hurst, William Jeffery;Ou, Boxin
通讯作者：
Ou, Boxin
影响因子：
6.1
作者：
Jalil, Abbe Maleyki Mhd;Ismail, Amin;Kamaruddin, Syed Hasbullah Syed
通讯作者：
Kamaruddin, Syed Hasbullah Syed