Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.
Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.
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DOI：
10.1016/j.mtbio.2023.100560
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发表时间：
2023-04
影响因子：
8.2
通讯作者：
Tang, Tzu-Chieh
中科院分区：
文献类型：
--
作者：
Jo, Charles;Zhang, Jing;Tam, Jenny M.;Church, George M.;Khalil, Ahmad S.;Segre, Daniel;Tang, Tzu-Chieh
丝状真菌通过与生长和腐烂的菌群及其组成微生物群的相互作用，驱动全球生态系统的碳和养分循环。丝状真菌中进化出的显着的代谢多样性、分泌能力和纤维状菌丝结构已在商业运营中得到越来越多的利用。菌丝发酵的工业潜力涵盖酶和生物活性化合物的发现和生物生产、食品和材料生产的脱碳、环境修复和提高农业生产。尽管霉菌和蘑菇对生态学和生物技术产生了根本性的影响，但迄今为止，霉菌和蘑菇尚未以与其他工业细胞工厂（例如大肠杆菌、酿酒酵母和 Komagataella phaffii）可比的方式与合成生物学产生显着的交叉。在这篇综述中，我们总结了一套合成生物学和计算工具，用于丝状真菌的挖掘、工程和优化，作为生物生产底盘。基因工程、诱变、实验进化和计算建模等方法的组合可用于解决现有产业和新兴产业中的菌株开发瓶颈。这些问题包括菌丝体生长速度慢、产量低、替代原料生长不理想以及下游纯化困难。在生物制造领域，我们详细介绍了之前通过针对蛋白质加工和分泌途径、菌丝形态发生和转录控制来改善关键瓶颈的努力。将合成生物学实践引入霉菌和蘑菇的隐藏世界将有助于扩大有限的宿主生物体范围，从而实现商业上可行且环境可持续的酶、化学品、治疗剂、食品和未来材料的生物生产。
Filamentous fungi drive carbon and nutrient cycling across our global ecosystems, through its interactions with growing and decaying flora and their constituent microbiomes. The remarkable metabolic diversity, secretion ability, and fiber-like mycelial structure that have evolved in filamentous fungi have been increasingly exploited in commercial operations. The industrial potential of mycelial fermentation ranges from the discovery and bioproduction of enzymes and bioactive compounds, the decarbonization of food and material production, to environmental remediation and enhanced agricultural production. Despite its fundamental impact in ecology and biotechnology, molds and mushrooms have not, to-date, significantly intersected with synthetic biology in ways comparable to other industrial cell factories (e.g. Escherichia coli,Saccharomyces cerevisiae, and Komagataella phaffii). In this review, we summarize a suite of synthetic biology and computational tools for the mining, engineering and optimization of filamentous fungi as a bioproduction chassis. A combination of methods across genetic engineering, mutagenesis, experimental evolution, and computational modeling can be used to address strain development bottlenecks in established and emerging industries. These include slow mycelium growth rate, low production yields, non-optimal growth in alternative feedstocks, and difficulties in downstream purification. In the scope of biomanufacturing, we then detail previous efforts in improving key bottlenecks by targeting protein processing and secretion pathways, hyphae morphogenesis, and transcriptional control. Bringing synthetic biology practices into the hidden world of molds and mushrooms will serve to expand the limited panel of host organisms that allow for commercially-feasible and environmentally-sustainable bioproduction of enzymes, chemicals, therapeutics, foods, and materials of the future.
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DOI：
10.1007/978-3-030-29541-7_7
发表时间：
2019-09-27
期刊：
Grand Challenges in Fungal Biotechnology
影响因子：
--
作者：
Arnau J;Yaver D;Hjort CM
通讯作者：
Hjort CM
影响因子：
3.4
作者：
Leonard CA;Brown SD;Hayman JR
通讯作者：
Hayman JR
影响因子：
2.7
作者：
Brandl, Julian;Andersen, Mikael R.
通讯作者：
Andersen, Mikael R.
影响因子：
--
作者：
Almpani-Lekka D;Pfeiffer S;Schmidts C;Seo SI
通讯作者：
Seo SI
影响因子：
4.6
作者：
Aminian-Dehkordi, Javad;Mousavi, Seyyed Mohammad;Marashi, Sayed-Amir
通讯作者：
Marashi, Sayed-Amir