Use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions.
Use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions.
复制标题
使用复制板测定快速评估唾液蛋白-细菌相互作用。
DOI:
10.1111/j.1399-302x.1992.tb00021.x
复制
发表时间:
1992
影响因子:
--
通讯作者:
Levine,MJ
中科院分区:
文献类型:
--
作者:
Tseng,CC;Scannapieco,FA;Levine,MJ
A replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with125I‐amylase or125I ‐proline‐rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains ofStreptococcus gordoniiandActinomyces viscosusbound amylase, and strains ofA. viscosusbound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.
登录
查看更多内容
DOI:
--
发表时间:
1969
期刊:
Biochimica et Biophysica Acta
影响因子:
--
作者:
Michael J. Levine;J. Weill;S. A. Ellison
通讯作者:
S. A. Ellison
DOI:
10.1016/s0006-291x(05)80900-3
发表时间:
1990
影响因子:
3.1
作者:
Scannapieco,FA;Bhandary,K;Ramasubbu,N;Levine,MJ
通讯作者:
Levine,MJ
DOI:
10.1042/bj2340043
发表时间:
1986
期刊:
The Biochemical journal
影响因子:
--
作者:
Bergey,EJ;Levine,MJ;Reddy,MS;Bradway,SD;Al-Hashimi,I
通讯作者:
Al-Hashimi,I
影响因子:
4.2
作者:
D. I. Hay;R. Gibbons;D. Spinell
通讯作者:
D. Spinell
影响因子:
3.5
作者:
S. Ebisu;H. Fukuhara;H. Okada
通讯作者:
H. Okada