Effect of FOXP2 transcription factor on immune infiltration of thyroid cancer and its potential clinical value.
Effect of FOXP2 transcription factor on immune infiltration of thyroid cancer and its potential clinical value.
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DOI：
10.3389/fimmu.2022.982812
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
--
作者：
某些甲状腺癌患者的临床结果并不总是令人满意。探讨叉头盒家族对甲状腺癌免疫细胞浸润及肿瘤微环境的影响。研究FOXP2在肿瘤侵袭和复发中的作用。首次采用TIMER和GEPIA比较FOXPs在多种人类癌症正常组织和癌组织中的表达。在匹配的甲状腺癌和邻近的正常组织，以及甲状腺癌细胞系和正常甲状腺细胞的一组中，用定量Real - Time-PCR和Western blot验证了数据库的结果。采用GEPIA平台探讨FOXPs作为预后指标的可能性。然后用TISIBD和UACLCAN来评估FOXPs对淋巴结转移和肿瘤分期的影响。首先采用GEPIA分析分析FOXPs与肿瘤免疫浸润细胞的相关性，然后根据肿瘤纯度纳入TIMER数据集进行标准化。FOXPs的不同成员在不同肿瘤组织中的表达存在差异。与匹配的正常组织相比，甲状腺癌组织中FOXP1、FOXP2水平较低，FOXP3、FOXP4水平较高。FOXP2、FOXP4与免疫侵袭呈负相关，FOXP1和FOXP3呈正相关。FOXPs与多种免疫细胞有显著的相关性。更重要的是，只有FOXP2对复发和肿瘤分期有显著影响。FOXP2作为免疫调节因子，其下调可能影响肿瘤微环境和免疫细胞浸润，增强肿瘤免疫逃逸，促进甲状腺癌复发。FOXP2可能是一种新的潜在诊断和预后指标。
The clinical outcomes are not always favorable in certain thyroid cancer patients. The effect of Forkhead-box family on immune cells infiltration and tumor microenvironment in thyroid cancer was explored. The role of FOXP2 in tumor invasion and recurrence was investigated consequently. TIMER and GEPIA were firstly employed to compare FOXPs expression in normal and cancer tissues from multiple human cancers. The results from database were confirmed by quantitative Real Time-PCR and Western blot in matched thyroid cancer and adjacent normal tissues, in addition to a panel of thyroid cancer cell lines and normal thyroid cell. GEPIA platform was employed to discover the possibility of FOXPs as prognostic indicator. TISIBD and UACLCAN were then employed to estimate the influence of FOXPs on lymph node metastasis and tumor staging. GEPIA analysis was initially employed to analyze correlation of FOXPs and tumor immune infiltrating cells, and TIMER dataset was then included for standardization according to tumor purity. Different member of FOXPs showed divergence in expression in various cancer tissues. Lower FOXP1, FOXP2 and higher FOXP3, FOXP4 levels could be identified in thyroid cancer tissues when compared with matched normal tissue. There was an inverse correlation between FOXP2, FOXP4 and immune invasion, whereas FOXP1 and FOXP3 were positively correlated. FOXPs showed remarkable correlations with multiply immune cells. More importantly, only FOXP2 showed the significant effect on recurrence and tumor staging. As immune regulatory factor, the reduction of FOXP2 may affect tumor microenvironments and immune cells infiltration, enhance tumor immune escape, and promote recurrence of thyroid cancer. FOXP2 could be a new potential diagnostic and prognostic marker.
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