Methamphetamine regulation of sulfotransferase 1A1 and 2A1 expression in rat brain sections.
Methamphetamine regulation of sulfotransferase 1A1 and 2A1 expression in rat brain sections.
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DOI：
10.1016/j.neuro.2012.09.010
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发表时间：
2013-01
期刊：
影响因子：
3.4
通讯作者：
Chen, Guangping
中科院分区：
文献类型：
--
作者：
Zhou, Tianyan;Huang, Chaoqun;Chen, Yue;Xu, Jiaojiao;Shanbhag, Preeti Devaraya;Chen, Guangping
磺基转移酶催化的硫酸化调节各种神经递质/激素的生物活性并使异生物质解毒。大鼠磺基转移酶rSULT 1A 1催化神经递质和异生酚类化合物的硫酸化。rSULT 2A 1催化羟基类固醇和异生物质醇类化合物的硫酸化。本研究采用Western blot和real-time RT-PCR方法，研究甲基苯丙胺对大鼠小脑、额叶皮质、海马和纹状体rSULT 1A 1和rSULT 2A 1蛋白和mRNA表达的影响。给药1天后，仅在小脑中观察到rSULT 1A 1的显著诱导;在小脑、额叶皮质和海马中显著诱导rSULT 2A 1。暴露7天后，rSULT 1A 1在小脑、额叶皮质和海马中被诱导，而rSULT 2A 1在所有四个区域中被显著诱导。Western blot结果与实时荧光定量PCR结果一致，表明诱导发生在基因转录水平。结果表明，rSULT 1A 1和rSULT 2A 1在大鼠额叶皮质、小脑、纹状体和海马中表达。rSULT 1A 1和rSULT 2A 1在大鼠脑切片中可被甲基苯丙胺以时间依赖的方式诱导。在大鼠脑切片中，甲基苯丙胺对rSULT 2A 1的诱导性高于rSULT 1A 1。甲基苯丙胺诱导活性的顺序为小脑>额叶皮质、海马>纹状体。这些结果表明，甲基苯丙胺可以影响rSULT 1A 1和rSULT 2A 1在不同脑区的生理功能。
Sulfotransferase catalyzed sulfation regulates the biological activities of various neurotransmitters/hormones and detoxifies xenobiotics. Rat sulfotransferase rSULT1A1 catalyzes the sulfation of neurotransmitters and xenobiotic phenolic compounds. rSULT2A1 catalyzes the sulfation of hydroxysteroids and xenobiotic alcoholic compounds. In this work, Western blot and real-time RT-PCR were used to investigate the effect of methamphetamine on rSULT1A1 and rSULT2A1 protein and mRNA expression in rat cerebellum, frontal cortex, hippocampus, and striatum. After 1-day treatment, significant induction of rSULT1A1 was observed only in the cerebellum; rSULT2A1 was induced significantly in the cerebellum, frontal cortex, and hippocampus. After 7-days of exposure, rSULT1A1 was induced in the cerebellum, frontal cortex, and hippocampus, while rSULT2A1 was induced significantly in all four regions. Western blot results agreed with the real-time RT-PCR results, suggesting that the induction occurred at the gene transcriptional level. Results indicate that rSULT1A1 and rSULT2A1 are expressed in rat frontal cortex, cerebellum, striatum, and hippocampus. rSULT1A1 and rSULT2A1are inducible by methamphetamine in rat brain sections in a time dependable manner. rSULT2A1 is more inducible than rSULT1A1 by methamphetamine in rat brain sections. Induction activity of methamphetamine is in the order of cerebellum > frontal cortex, hippocampus > striatum. These results suggest that the physiological functions of rSULT1A1 and rSULT2A1 in different brain regions can be affected by methamphetamine.
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影响因子：
3.9
作者：
Fang, HL;Abdolalipour, M;Runge-Morris, M
通讯作者：
Runge-Morris, M
DOI：
10.1016/s0169-328x(03)00257-2
发表时间：
2003-09-10
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Kabbaj, M;Yoshida, S;Sato, M
通讯作者：
Sato, M
影响因子：
3.6
作者：
Gaytan, O;Swann, A;Dafny, N
通讯作者：
Dafny, N
影响因子：
10.6
作者：
Gold, Mark S.;Kobeissy, Firas H.;Wang, Kevin K. W.;Merlo, Lisa J.;Bruijnzeel, Adriaan W.;Krasnova, Irina N.;Cadet, Jean Lud
通讯作者：
Cadet, Jean Lud
影响因子：
3.8
作者：
Alnouti, Yazen;Klaassen, Curtis D.
通讯作者：
Klaassen, Curtis D.