Formulation and in vitro characterization of composite biodegradable magnetic nanoparticles for magnetically guided cell delivery.
Formulation and in vitro characterization of composite biodegradable magnetic nanoparticles for magnetically guided cell delivery.
复制标题
DOI：
10.1007/s11095-012-0675-y
复制
发表时间：
2012-05
影响因子：
3.7
通讯作者：
Levy, Robert J.
中科院分区：
文献类型：
--
作者：
Chorny, Michael;Alferiev, Ivan S.;Fishbein, Ilia;Tengood, Jillian E.;Folchman-Wagner, Zoe;Forbes, Scott P.;Levy, Robert J.
关键词：
用磁响应纳米颗粒（MNP）修饰的细胞可以为新的靶向治疗策略提供基础。然而，需要改进MNP设计和细胞处理方案，以提供与可接受的细胞活力和功能平衡的足够的磁性。本研究的重点是选择控制可生物降解的聚合物为基础的MNP在培养的内皮细胞的摄取和细胞相容性的变量。使用磁铁矿和聚乳酸作为结构组分形成了荧光标记的MNP。与非磁性对照条件相比，相对于细胞活力，通过荧光法研究了它们的磁驱动沉降和摄取。使用绿色荧光蛋白报告基因检查了表面活化的MNP与复制缺陷型腺病毒（Ad）形成亲和复合物用于伴随磁性细胞加载实现的转导的效用。一个高梯度磁场是必不可少的沉淀和细胞结合的白蛋白稳定的MNP，后者是限速的MNP加载过程。可实现高达160 pg氧化铁/细胞的细胞负载，细胞活力> 90%。磁性驱动的MNP-Ad复合物的摄取可以提供高水平的转基因表达，可能用于组合的细胞/基因疗法。用于靶向递送应用的磁响应性内皮细胞可以使用复合可生物降解的MNP快速有效地获得。
Cells modified with magnetically responsive nanoparticles (MNP) can provide the basis for novel targeted therapeutic strategies. However, improvements are required in the MNP design and cell treatment protocols to provide adequate magnetic properties in balance with acceptable cell viability and function. This study focused on select variables controlling the uptake and cell compatibility of biodegradable polymer-based MNP in cultured endothelial cells. Fluorescent-labeled MNP were formed using magnetite and polylactide as structural components. Their magnetically driven sedimentation and uptake were studied fluorimetrically relative to cell viability in comparison to non-magnetic control conditions. The utility of surface-activated MNP forming affinity complexes with replication-deficient adenovirus (Ad) for transduction achieved concomitantly with magnetic cell loading was examined using the green fluorescent protein reporter. A high-gradient magnetic field was essential for sedimentation and cell binding of albumin-stabilized MNP, the latter being rate-limiting in the MNP loading process. Cell loading up to 160 pg iron oxide per cell was achievable with cell viability >90%. Magnetically driven uptake of MNP-Ad complexes can provide high levels of transgene expression potentially useful for a combined cell/gene therapy. Magnetically responsive endothelial cells for targeted delivery applications can be obtained rapidly and efficiently using composite biodegradable MNP.
登录
查看更多内容
影响因子：
4.9
作者：
Tresilwised, Nittaya;Pithayanukul, Pimolpan;Plank, Christian
通讯作者：
Plank, Christian
影响因子：
12.4
作者：
Chorny, Michael;Fishbein, Ilia;Levy, Robert J.
通讯作者：
Levy, Robert J.
影响因子：
11.3
作者：
Kyrtatos, Panagiotis G.;Lehtolainen, Pauliina;Lythgoe, Mark F.
通讯作者：
Lythgoe, Mark F.
影响因子：
4.9
作者：
Chorny M;Fishbein I;Alferiev I;Levy RJ
通讯作者：
Levy RJ
影响因子：
2.8
作者：
Shimizu, Kazunori;Ito, Akira;Honda, Hiroyuki
通讯作者：
Honda, Hiroyuki