Bioengineered Kidney Models: Methods and Functional Assessments.
Bioengineered Kidney Models: Methods and Functional Assessments.
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DOI：
10.1093/function/zqab026
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Morizane R
中科院分区：
文献类型：
--
作者：
Rizki-Safitri A;Traitteur T;Morizane R
生物工程肾脏的研究已被广泛进行，由于其潜在的临床前试验和再生医学。已经开发了各种途径和方法来改善生物工程肾脏的结构和功能。功能特性的评估证实了生物工程肾脏对于多用途翻译应用的充分性。本文就近十年来肾脏生物工程的研究进展作一综述。我们从PubMed和Mendeley中检索到84篇原始文献，关键词为肾类器官或肾组织工程。根据其方法将其分为5组：肾脏的去细胞化/再细胞化、肾脏细胞的再聚集、肾脏类器官、支架中的肾脏和芯片上的肾脏。通过滤过、肾小管重吸收/分泌、激素产生和肾毒性对这些模型进行生理学评估。我们发现，生物工程肾脏模型已经从简单的细胞培养发展到多细胞系统，以概括肾脏功能和疾病。同时，只有大约50%的研究对他们的肾脏模型进行了功能评估。包括细胞组成和组织在内的因素可能会改变生物工程肾脏中生理评估的适用性。结合最近的技术，生理评估重要地有助于改善生物工程肾脏模型，以修复和重建受损的肾脏。
Investigations into bioengineering kidneys have been extensively conducted owing to their potential for preclinical assays and regenerative medicine. Various approaches and methods have been developed to improve the structure and function of bioengineered kidneys. Assessments of functional properties confirm the adequacy of bioengineered kidneys for multipurpose translational applications. This review is to summarize the studies performed in kidney bioengineering in the past decade. We identified 84 original articles from PubMed and Mendeley with keywords of kidney organoid or kidney tissue engineering. Those were categorized into 5 groups based on their approach: de-/recellularization of kidney, reaggregation of kidney cells, kidney organoids, kidney in scaffolds, and kidney-on-a-chip. These models were physiologically assessed by filtration, tubular reabsorption/secretion, hormone production, and nephrotoxicity. We found that bioengineered kidney models have been developed from simple cell cultures to multicellular systems to recapitulate kidney function and diseases. Meanwhile, only about 50% of these studies conducted functional assessments on their kidney models. Factors including cell composition and organization are likely to alter the applicability of physiological assessments in bioengineered kidneys. Combined with recent technologies, physiological assessments importantly contribute to the improvement of the bioengineered kidney model toward repairing and refunctioning the damaged kidney.
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DOI：
10.1097/mat.0000000000000723
发表时间：
2018
期刊：
ASAIO journal (American Society for Artificial Internal Organs : 1992)
影响因子：
--
作者：
Ferrell N;Cheng J;Miao S;Roy S;Fissell WH
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影响因子：
23.9
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Freedman BS
影响因子：
11.1
作者：
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Xinaris, Christodoulos
影响因子：
16.6
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Calandrini, Camilla;Schutgens, Frans;Drost, Jarno
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Drost, Jarno
影响因子：
10
作者：
Ali, Mohamed;Kumar, Anil P. R.;Lee, Sang Jin
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