Genetic basis of autoimmune sialadenitis in MRL/lpr lupus-prone mice: additive and hierarchical properties of polygenic inheritance.
Genetic basis of autoimmune sialadenitis in MRL/lpr lupus-prone mice: additive and hierarchical properties of polygenic inheritance.
复制标题
MRL/lpr 狼疮易感小鼠中自身免疫性唾液腺炎的遗传基础：多基因遗传的加性和分层特性。
作者：
M. Nishihara;M. Terada;J. Kamogawa;Y. Ohashi;S. Mori;S. Nakatsuru;Y. Nakamura;M. Nose
目的
目的明确MRL/MpJ-lpr/lpr（MRL/lpr）狼疮易感小鼠自身免疫性涎腺炎的遗传方式，并确定易感基因位点。
方法
制备MRL/lpr、C3 H/HeJ-lpr/lpr（C3 H/lpr）、（MRL/lpr × C3 H/lpr）F1互交和MRL/lpr ×（MRL/lpr × C3 H/lpr）F1回交小鼠，并通过组织病理学分级分析个体小鼠的涎腺炎。对回交小鼠基因组DNA进行简单序列长度多态性分析，确定与涎腺炎高度相关的多态性微卫星标记为涎腺炎易感位点。
结果
4个与涎腺炎相关的易感基因位点分别定位在第10、18、4和1号染色体上。这些位点在涎腺炎的发展中表现出加性和层次性。
结论
结果表明MRL/lpr小鼠的涎腺炎是多基因遗传的，可能涉及等位基因多态性。
OBJECTIVE
To clarify the mode of inheritance of autoimmune sialadenitis in MRL/MpJ-lpr/lpr (MRL/lpr) lupus-prone mice and identify the susceptibility loci.
METHODS
MRL/lpr, C3H/HeJ-lpr/lpr (C3H/lpr), (MRL/lpr x C3H/lpr)F1 intercross, and MRL/lpr x (MRL/lpr x C3H/lpr)F1 backcross mice were prepared, and sialadenitis in individual mice was analyzed by histopathologic grading. The genomic DNA of the backcross mice was examined by simple sequence-length polymorphism analysis, and the highly associated polymorphic microsatellite markers with sialadenitis were determined as sialadenitis susceptibility loci.
RESULTS
Four susceptible gene loci recessively associated with sialadenitis were mapped on chromosomes 10, 18, 4, and 1, respectively. These loci manifested additive and hierarchical properties in the development of sialadenitis.
CONCLUSION
The results indicate that sialadenitis in MRL/lpr mice is under the control of polygenic inheritance, possibly involving allelic polymorphism.
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MRL-Fas lpr 和 C57BL/6-Faslpr 小鼠中易产生自身免疫的位点。
DOI：
10.1172/jci1817
发表时间：
1998
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Vidal,S;Kono,DH;Theofilopoulos,AN
通讯作者：
Theofilopoulos,AN
影响因子：
--
作者：
Wilson,RW;Provost,TT;Bias,WB;Alexander,EL;Edlow,DW;Hochberg,MC;Stevens,MB;Arnett,FC
通讯作者：
Arnett,FC
影响因子：
32.4
作者：
MOREL, L;RUDOFSKY, UH;WAKELAND, EK
通讯作者：
WAKELAND, EK
影响因子：
4.4
作者：
J. Reveille;Miriam J. Macleod;K. Whittington;F. Arnett
通讯作者：
J. Reveille;Miriam J. Macleod;K. Whittington;F. Arnett
影响因子：
--
作者：
HOFFMAN, RW;ALSPAUGH, MA;WALKER, SE
通讯作者：
WALKER, SE