Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis).
Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis).
复制标题
甜橙（Citrus sinensis）果实发育和成熟过程中转录组的变化
DOI：
10.1186/1471-2164-13-10
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发表时间：
2012-01-10
期刊：
影响因子：
4.4
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Yu K;Xu Q;Da X;Guo F;Ding Y;Deng X
研究了甜橙品种安柳（WT）及其红肉突变体红安柳（MT）果肉转录组的差异，以了解果实发育和成熟过程中基因表达的动态和差异表达。结果利用Illumina测序平台对WT和MT的四个发育阶段的转录组进行了采样。在MT和WT中分别检测到19,440和18,829个基因。分层聚类分析发现，所有检测到的基因共有24种表达模式，其中20种基因在MT和WT之间共有。在WT中，89%以上的基因在果实发育和成熟过程中表现出差异表达。对胁迫的反应是发生在果实发育和成熟过程中差异调节最大的过程。结论对甜橙果实发育和成熟过程中的转录组变化进行了描述，并对野生型和红肉突变体基因表达差异进行了动态观察。
BackgroundThe transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening.ResultsThe transcriptomes of WT and MT were sampled at four developmental stages using an Illumina sequencing platform. A total of 19,440 and 18,829 genes were detected in MT and WT, respectively. Hierarchical clustering analysis revealed 24 expression patterns for the set of all genes detected, of which 20 were in common between MT and WT. Over 89% of the genes showed differential expression during fruit development and ripening in the WT. Functional categorization of the differentially expressed genes revealed that cell wall biosynthesis, carbohydrate and citric acid metabolism, carotenoid metabolism, and the response to stress were the most differentially regulated processes occurring during fruit development and ripening.ConclusionA description of the transcriptomic changes occurring during fruit development and ripening was obtained in sweet orange, along with a dynamic view of the gene expression differences between the wild type and a red fleshed mutant.
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影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
2.4
作者：
Licciardello, Concetta;Russo, Maria Patrizia;Recupero, Reforgiato Giuseppe
通讯作者：
Recupero, Reforgiato Giuseppe
影响因子：
6.9
作者：
Alquézar B;Zacarías L;Rodrigo MJ
通讯作者：
Rodrigo MJ
影响因子：
5.1
作者：
Cercos, Manuel;Soler, Guillermo;Talon, Manuel
通讯作者：
Talon, Manuel
影响因子：
4.1
作者：
Bartolozzi, F;Bertazza, G;Cristoferi, G
通讯作者：
Cristoferi, G