Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development.
Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development.
复制标题
DOI：
10.1186/1471-2164-8-429
复制
发表时间：
2007-11-22
期刊：
影响因子：
4.4
通讯作者：
Cramer GR
中科院分区：
文献类型：
--
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
葡萄浆果的发育是一个动态过程，涉及一系列复杂的分子遗传和生化变化，分为三个主要阶段，在初始浆果生长（I期）中，由于细胞分裂和后续细胞膨胀而沿着Sigmoidal的生长曲线增加有机酸（主要是苹果酸和茶叶酸盐），单宁和羟基菌积聚到峰值水平（II期）。 véraison（成熟的开始）标志着第三个主要阶段（第三阶段）的开始，其中第二个浆果在第二个乙状结肠生长下，由于额外的中核细胞膨胀，花青素色素积累浆果颜色的积累，钙蛋白质的颜色，挥发性化合物的积累，糖的峰值积累（主要是葡萄糖和果糖），以及有机酸积累的下降。负责控制浆果发育的转录网络，使用Affymetrixgenechip®寡核苷酸寡核苷酸微阵列的VINECHICS cabernet Sauvignon进行了mRNA表达分析。通过修饰的E-L系统定义），通过Véraison（E-L阶段34和35），到成熟的浆果（E-L阶段36和38）与MRNA表达分析并行分析，以了解转录调节过程对特定代谢产物的影响，最终影响葡萄酒的有机特性。
在浆果发育过程中，发现整个水果组织的平均含量为74.5％，其中有14,470个单基因的vision微阵列中所示的问题。浆果发育（28％的转录本，4,151个单基因，在mRNA表达中显着（≥2倍）差异），说明了发育的动态性质过程。修改的E-L系统。钙隔离，传输和信号，细胞壁代谢介导膨胀，成熟和软化，类黄酮代谢和转运，有机和氨基酸代谢，己糖糖和磷酸盐代谢和转运，淀粉代谢，淀粉代谢，光合作用，光合作用，昼夜节律，转录因子，脱甲酸（ABA）生物合成和钙信号基因的mRNA表达模式确定了候选者可能参与关键发育事件的进展，例如véraison和潜在的候选基因，例如浆果细胞中的生长素分析，香气化合物产生以及途径调节类黄酮化合物的途径。模式表明存在从蔬菜到成熟浆果所调用的葡萄糖和三糖产生的替代途径。
这些结果揭示了在葡萄浆果发育的七个阶段发生的转录组动力学的第一张高分辨率图片。在更重要的葡萄酒品种中，该分析确定了一组以前未知的基因与水果发展相关的步骤，现在可以进行功能测试。
Grape berry development is a dynamic process that involves a complex series of molecular genetic and biochemical changes divided into three major phases. During initial berry growth (Phase I), berry size increases along a sigmoidal growth curve due to cell division and subsequent cell expansion, and organic acids (mainly malate and tartrate), tannins, and hydroxycinnamates accumulate to peak levels. The second major phase (Phase II) is defined as a lag phase in which cell expansion ceases and sugars begin to accumulate. Véraison (the onset of ripening) marks the beginning of the third major phase (Phase III) in which berries undergo a second period of sigmoidal growth due to additional mesocarp cell expansion, accumulation of anthocyanin pigments for berry color, accumulation of volatile compounds for aroma, softening, peak accumulation of sugars (mainly glucose and fructose), and a decline in organic acid accumulation. In order to understand the transcriptional network responsible for controlling berry development, mRNA expression profiling was conducted on berries of V. vinifera Cabernet Sauvignon using the Affymetrix GeneChip® Vitis oligonucleotide microarray ver. 1.0 spanning seven stages of berry development from small pea size berries (E-L stages 31 to 33 as defined by the modified E-L system), through véraison (E-L stages 34 and 35), to mature berries (E-L stages 36 and 38). Selected metabolites were profiled in parallel with mRNA expression profiling to understand the effect of transcriptional regulatory processes on specific metabolite production that ultimately influence the organoleptic properties of wine. Over the course of berry development whole fruit tissues were found to express an average of 74.5% of probes represented on the Vitis microarray, which has 14,470 Unigenes. Approximately 60% of the expressed transcripts were differentially expressed between at least two out of the seven stages of berry development (28% of transcripts, 4,151 Unigenes, had pronounced (≥2 fold) differences in mRNA expression) illustrating the dynamic nature of the developmental process. The subset of 4,151 Unigenes was split into twenty well-correlated expression profiles. Expression profile patterns included those with declining or increasing mRNA expression over the course of berry development as well as transient peak or trough patterns across various developmental stages as defined by the modified E-L system. These detailed surveys revealed the expression patterns for genes that play key functional roles in phytohormone biosynthesis and response, calcium sequestration, transport and signaling, cell wall metabolism mediating expansion, ripening, and softening, flavonoid metabolism and transport, organic and amino acid metabolism, hexose sugar and triose phosphate metabolism and transport, starch metabolism, photosynthesis, circadian cycles and pathogen resistance. In particular, mRNA expression patterns of transcription factors, abscisic acid (ABA) biosynthesis, and calcium signaling genes identified candidate factors likely to participate in the progression of key developmental events such as véraison and potential candidate genes associated with such processes as auxin partitioning within berry cells, aroma compound production, and pathway regulation and sequestration of flavonoid compounds. Finally, analysis of sugar metabolism gene expression patterns indicated the existence of an alternative pathway for glucose and triose phosphate production that is invoked from véraison to mature berries. These results reveal the first high-resolution picture of the transcriptome dynamics that occur during seven stages of grape berry development. This work also establishes an extensive catalog of gene expression patterns for future investigations aimed at the dissection of the transcriptional regulatory hierarchies that govern berry development in a widely grown cultivar of wine grape. More importantly, this analysis identified a set of previously unknown genes potentially involved in critical steps associated with fruit development that can now be subjected to functional testing.
登录
查看更多内容
影响因子：
--
作者：
CHERNOFF, H;LEHMANN, EL
通讯作者：
LEHMANN, EL
影响因子：
7.4
作者：
Davies, C;Boss, PK;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
7.4
作者：
Davies, C;Robinson, SP
通讯作者：
Robinson, SP
DOI：
10.1111/j.1755-0238.1995.tb00086.x
发表时间：
1995-01-01
影响因子：
2.8
作者：
Coombe, B. G.
通讯作者：
Coombe, B. G.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y