Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models.
Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models.
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DOI：
10.3390/v8120322
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发表时间：
2016-11-29
期刊：
影响因子：
--
通讯作者：
Tanuri A
中科院分区：
文献类型：
--
作者：
Delvecchio R;Higa LM;Pezzuto P;Valadão AL;Garcez PP;Monteiro FL;Loiola EC;Dias AA;Silva FJ;Aliota MT;Caine EA;Osorio JE;Bellio M;O'Connor DH;Rehen S;de Aguiar RS;Savarino A;Campanati L;Tanuri A
子宫内的寨卡病毒（ZIKV）感染可能会导致小头畸形和其他先天性缺陷。由于迄今为止还没有具体的治疗方法，因此迫切需要发现能够抑制其病毒复制和有害作用的药物。氯喹被广泛用作抗疟药、抗炎药，并且还对多种病毒显示出抗病毒活性。在这里，我们发现氯喹在 Vero 细胞、人脑微血管内皮细胞、人神经干细胞和小鼠神经球中表现出针对 ZIKV 的抗病毒活性。我们证明氯喹可以在体外减少 ZIKV 感染的细胞数量，并抑制 ZIKV 感染促进的病毒产生和细胞死亡，而没有细胞毒性作用。此外，氯喹治疗可部分逆转 ZIKV 感染引起的小鼠神经球形态变化。
Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres.
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影响因子：
--
作者：
Gilmore, Edward C.;Walsh, Christopher A.
通讯作者：
Walsh, Christopher A.
影响因子：
3.8
作者：
Faye O;Freire CC;Iamarino A;Faye O;de Oliveira JV;Diallo M;Zanotto PM;Sall AA
通讯作者：
Sall AA
影响因子：
5.4
作者：
Harley, CA;Dasgupta, A;Wilson, DW
通讯作者：
Wilson, DW
影响因子：
30.3
作者：
Barrows NJ;Campos RK;Powell ST;Prasanth KR;Schott-Lerner G;Soto-Acosta R;Galarza-Muñoz G;McGrath EL;Urrabaz-Garza R;Gao J;Wu P;Menon R;Saade G;Fernandez-Salas I;Rossi SL;Vasilakis N;Routh A;Bradrick SS;Garcia-Blanco MA
通讯作者：
Garcia-Blanco MA
DOI：
10.1590/s0074-02762013000500010
发表时间：
2013-08-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Borges, Marcos Carvalho;Castro, Luiza Antunes;Fonseca, Benedito Antonio Lopes da
通讯作者：
Fonseca, Benedito Antonio Lopes da