Molecular detection and phylogenetic analyses of Wolbachia in natural populations of nine galling Aphid species
Molecular detection and phylogenetic analyses of Wolbachia in natural populations of nine galling Aphid species
复制标题
九种瘿蚜自然种群中沃尔巴克氏体的分子检测和系统发育分析
DOI：
10.1038/s41598-020-68925-z
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发表时间：
2020-07
影响因子：
4.6
通讯作者：
Yang Zixiang
中科院分区：
文献类型：
--
作者：
Ren Weibin;Wei Hongyuan;Yang Ying;Shao Shuxia;Wu Haixia;Chen Xiaoming;Yang Zixiang
Wolbachia是节肢动物中最丰富的兼性细胞内共生体之一。它以不同的方式改变宿主生物学，包括诱导生殖操纵，营养供应者的关联和对病原体的保护。蚜虫是一类具有复杂生活史、有性生殖和无性生殖交替、寄主间转移等生物学特性的昆虫。沃尔巴克氏体广泛存在于昆虫的许多目中，但迄今为止，对蚜虫体内沃尔巴克氏体的研究有限。瘿蚜是指在特定的生活史阶段在寄主植物上产生瘿的一类蚜虫。在这项研究中，15个自然种群代表9种虫瘿蚜虫进行了分析，使用物种特异性引物对沃尔巴克氏体的存在。沃尔巴克氏体存在于瘿蚜虫是相当低的，蚜虫种群之间的差异显着。在我们分析的15个人群中，只有3个人群可检测到沃尔巴克氏体，总感染率为20%。从杜鹃蚜（Kaburagia rhusicola）和中华白蛾（Schlechtendalia chinensis）上分离到两个沃尔巴克氏体菌株O和B。O菌株是首次在蚜虫中发现的，它可能与生活在封闭微环境中的蚜虫的生活阶段有关，具有不同于自由生活的蚜虫的特定生存策略。
Wolbachia is one of the most abundant facultative intracellular symbionts in arthropods. It alters host biology in diverse ways, including the induction of reproductive manipulation, association of nutrient supplier and protection against pathogens. Aphids are a group of insects which exhibit interesting biological characteristics such as complex life cycles, alteration of sexual and asexual reproduction and shifts between two different hosts. Wolbachia is widely present in many orders of insects, but so far limited studies on Wolbachia in aphids have been carried out. Galling aphids are a group of aphids that induce galls on their primary host plants at specific life stage. In this study, 15 natural populations representing nine galling aphid species were analyzed for the presence of Wolbachia using species-specific primer pairs. Wolbachia presence in galling aphids was quite low and varied significantly among aphid populations. Only three of the 15 populations we analyzed had detectable Wolbachia and the overall infection rate was 20%. Two Wolbachia strains, O and B, were identified from the galling aphids Kaburagia rhusicola and Schlechtendalia chinensis. Strain O was for the first time to be found in aphids, and it is likely involved with the life stages of galling aphids living in closed microenvironments with specific survival strategies that are different from free-living aphids.
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影响因子：
2.6
作者：
Bing, Xiao-Li;Xia, Wen-Qiang;Gui, Jia-Dong;Yan, Gen-Hong;Wang, Xiao-Wei;Liu, Shu-Sheng
通讯作者：
Liu, Shu-Sheng
影响因子：
10.7
作者：
Reuter, M;Keller, L
通讯作者：
Keller, L
影响因子：
4.9
作者：
T. Tsuchida;R. Koga;H. Shibao;Tadao Matsumoto;T. Fukatsu
通讯作者：
T. Tsuchida;R. Koga;H. Shibao;Tadao Matsumoto;T. Fukatsu
DOI：
10.1098/rspb.2000.1139
发表时间：
2000-07-07
影响因子：
4.7
作者：
Werren, JH;Windsor, DM
通讯作者：
Windsor, DM
影响因子：
3.2
作者：
Gómez-Valero, L;Soriano-Navarro, M;Latorre, A
通讯作者：
Latorre, A