Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies.
Diversity and evolution of the Wolbachia endosymbionts of Bemisia (Hemiptera: Aleyrodidae) whiteflies.
复制标题
DOI：
10.1002/ece3.1126
复制
发表时间：
2014-07
影响因子：
2.6
通讯作者：
Liu, Shu-Sheng
中科院分区：
文献类型：
--
作者：
Bing, Xiao-Li;Xia, Wen-Qiang;Gui, Jia-Dong;Yan, Gen-Hong;Wang, Xiao-Wei;Liu, Shu-Sheng
关键词：
沃尔巴克氏体是迄今为止节肢动物中描述的最普遍的共生体。沃尔巴克氏体可以操纵宿主繁殖，为昆虫宿主提供营养，保护昆虫宿主免受病原病毒的侵害。到目前为止，已经确定了13个沃尔巴克氏体超级群。烟粉虱是一个复合体，包含超过28个形态上难以区分的隐种。该复合体的一些隐种是侵入性的。在这项研究中，我们报告了对中国13个省和澳大利亚一个州的54个种群的1658只昆虫及其近亲烟粉虱沃尔巴克氏体的全面调查。根据16S rRNA基因的PCR和测序结果，本地白蝇和入侵白蝇沃尔巴克氏体的总体感染率分别为79.6%和0.96%。通过对16S rRNA、groEL、gltA、hcpA和fbpA 5个分子标记基因的测序，发现了一个新的Wolbachia超群。数据显示，许多蛋白质编码基因在检测和分类新发现的沃尔巴克氏体超群方面存在局限性，从而对已知的沃尔巴克氏体MLST标准分析系统提出了挑战。此外，从白蝇中检出的其他沃尔巴克氏菌均聚集在b超群中。白蝇线粒体细胞色素氧化酶I亚基与沃尔巴克氏菌多序列分型基因的系统发育树不一致。此外，荧光原位杂交还在两种隐种的特殊细菌细胞外检测到沃尔巴克氏体，表明沃尔巴克氏体存在水平传播。我们的结果表明，沃尔巴克氏体的成员还远远没有被充分探索。
Wolbachia is the most prevalent symbiont described in arthropods to date. Wolbachia can manipulate host reproduction, provide nutrition to insect hosts and protect insect hosts from pathogenic viruses. So far, 13 supergroups of Wolbachia have been identified. The whitefly Bemisia tabaci is a complex containing more than 28 morphologically indistinguishable cryptic species. Some cryptic species of this complex are invasive. In this study, we report a comprehensive survey of Wolbachia in B. tabaci and its relative B. afer from 1658 insects representing 54 populations across 13 provinces of China and one state of Australia. Based on the results of PCR or sequencing of the 16S rRNA gene, the overall rates of Wolbachia infection were 79.6% and 0.96% in the indigenous and invasive Bemisia whiteflies, respectively. We detected a new Wolbachia supergroup by sequencing five molecular marker genes including 16S rRNA, groEL, gltA, hcpA, and fbpA genes. Data showed that many protein-coding genes have limitations in detecting and classifying newly identified Wolbachia supergroups and thus raise a challenge to the known Wolbachia MLST standard analysis system. Besides, the other Wolbachia strains detected from whiteflies were clustered into supergroup B. Phylogenetic trees of whitefly mitochondrial cytochrome oxidase subunit I and Wolbachia multiple sequencing typing genes were not congruent. In addition, Wolbachia was also detected outside the special bacteriocytes in two cryptic species by fluorescence in situ hybridization, indicating the horizontal transmission of Wolbachia. Our results indicate that members of Wolbachia are far from well explored.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
2.6
作者：
Baldo, Laura;Werren, John H.
通讯作者：
Werren, John H.
影响因子：
4.9
作者：
Frohlich, DR;Torres-Jerez, I;Brown, JK
通讯作者：
Brown, JK
影响因子：
10.7
作者：
Bryant, D;Moulton, V
通讯作者：
Moulton, V
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM