Identification and preliminary characterization of Hc-clec-160, a novel C-type lectin domain-containing gene of the strongylid nematode Haemonchus contortus.
Identification and preliminary characterization of Hc-clec-160, a novel C-type lectin domain-containing gene of the strongylid nematode Haemonchus contortus.
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捻转血矛线虫 C 型凝集素结构域基因 Hc-clec-160 的鉴定和初步表征
DOI：
10.1186/s13071-018-3005-3
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发表时间：
2018-07-20
影响因子：
3.2
通讯作者：
Du A
中科院分区：
文献类型：
--
作者：
Zhang L;Mou L;Chen X;Yang Y;Hu M;Li X;Suo X;Zhu XQ;Du A
背景：圆线虫寄生虫捻转血矛线虫在世界范围内引起家畜严重贫血。由于耐药性的存在以及对H.方法：采用RT-PCR方法发现一个新基因Hc-clec-160。通过qPCR测定相应线虫的整个不同生长期的Hc-clec-160和Ce-clec-160的转录水平。石蜡切片免疫荧光染色，确定该蛋白在蠕虫中的定位。扭曲为了监测Ce-clec-160的5 '侧翼区的启动子能力，使用显微注射。结果：RT-PCR扩增出Hc-clec-160全长cDNA，全长1224 bp。相应的基因包含12个外显子。其转录在雄性蠕虫中达到峰值。预测Hc-CLEC-160具有A型维勒布兰德因子（vWA）结构域和C型凝集素结构域。在C. elegans或石蜡切片实验。扭曲Ce-clec-160在C.结论：本实验在线虫中获得了一个新基因Hc-clec-160。使用模式生物C.优雅研究表明，Hc-clec-160具有与Ce-clec-160相似的特性，在该线虫的生长繁殖中起重要作用。
Background:The strongylid parasite Haemonchus contortus causes severe anemia in domestic animals worldwide. Effective preventive and therapeutical agents are lacking, because of drug resistance and that little is known about the molecular mechanism of the interaction between H. contortus and host cells.Methods:A new gene, Hc-clec-160, was discovered with RT-PCR. Transcriptional levels of Hc-clec-160 and Ce-clec-160 throughout different growth phases of corresponding nematodes were assayed by qPCR. Immunofluorescence staining of paraffin section were performed to determine the protein localization in adult worms of H. contortus. To monitor the promoter capacity of the 5'-flanking region of Ce-clec-160, micro-injection was used. Overexpression and RNAi constructs was carried out in the N2 strain of Caenorhabditis elegans to find out the gene function of Hc-clec-160.Results:The full-length cDNA of 1224 bp of Hc-clec-160 was cloned by RT-PCR. The corresponding gene contained twelve exons. Its transcripts peaked in male adult worms. Hc-CLEC-160 was predicted to have a Willebrand factor type A (vWA) domain and a C-type lectin domain. The proteins were not detected by expression in C. elegans or paraffin section experiments in adult of H. contortus. Knockdown of Ce-clec-160 expression in C. elegans by RNAi resulted in shortened body length and decreased brood size.Conclusions:In this experiment, a new gene Hc-clec-160 was obtained in H. contortus and its function was addressed using a model organism: C. elegans. Our study showed that Hc-clec-160 possesses characteristics similar to those of Ce-clec-160 and plays an important role in the growth and reproduction of this parasitic nematode.
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影响因子：
4.3
作者：
Meyer, Sandra;Tefsen, Boris;van Die, Irma
通讯作者：
van Die, Irma
影响因子：
6.7
作者：
Taylor CM;Martin J;Rao RU;Powell K;Abubucker S;Mitreva M
通讯作者：
Mitreva M
影响因子：
2.4
作者：
Loukas, A;Doedens, A;Maizels, RM
通讯作者：
Maizels, RM
甲状腺素在感染中的作用。
DOI：
10.1038/nrmicro2146
发表时间：
2009-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3
作者：
Young, AR;Meeusen, EN
通讯作者：
Meeusen, EN