Angiotensin-converting enzyme inhibitors attenuate propofol-induced pro-oxidative and antifibrinolytic effect in human endothelial cells.
Angiotensin-converting enzyme inhibitors attenuate propofol-induced pro-oxidative and antifibrinolytic effect in human endothelial cells.
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DOI：
10.1177/1470320316687197
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Chabielska E
中科院分区：
文献类型：
--
作者：
Wojewodzka-Zelezniakowicz M;Gromotowicz-Poplawska A;Kisiel W;Konarzewska E;Szemraj J;Ladny JR;Chabielska E
本研究旨在观察血浆和组织血管紧张素转换酶抑制剂(ACE-IS)对异丙酚诱导的血管内皮细胞功能障碍的影响，并探讨其作用机制。我们观察了异丙酚(50μM)、奎那普利拉和依那普利拉(10−5 M)对人脐静脉内皮细胞纤溶活性(t-PA、PAI-1、TAFI抗原水平)、氧化应激参数(H_2O_2和丙二醛抗原水平、超氧化物歧化酶和NADPH氧化酶基因水平)和一氧化氮生物利用度(NO_2/NO_3浓度和一氧化氮合酶基因表达水平)的影响。我们发现，在体外，两种ACE-IS都能促进相似的内皮纤溶特性，并降低氧化应激。单用异丙酚可增加血管内皮细胞释放抗纤溶因子和促氧化因子，并增加iNOS的表达。我们还发现，在ACE-IS预孵育后，异丙酚与HUVECs共同孵育会导致异丙酚的抗纤溶和促氧化作用减弱，这一作用在ACE-IS预孵育后相似。这一观察结果表明，所研究的ACE-IS对异丙酚引起的内皮细胞功能障碍具有保护作用，而不依赖于血流动力学。
The aim of this study was to investigate the effects of plasma and tissue angiotensin-converting enzyme inhibitors (ACE-Is) against propofol-induced endothelial dysfunction and to elucidate the involved mechanisms in vitro. We examined the effects of propofol (50 μM), quinaprilat and enalaprilat (10−5 M) on fibrinolysis (t-PA, PAI-1, TAFI antigen levels), oxidative stress parameters (H2O2 and MDA antigen levels and SOD and NADPH oxidase mRNA levels) and nitric oxide bioavailability (NO2/NO3 concentration and NOS expression at the level of mRNA) in human umbilical vein endothelial cells (HUVECs). We found that both ACE-Is promoted similar endothelial fibrinolytic properties and decreased oxidative stress in vitro. Propofol alone increased the release of antifibrinolytic and pro-oxidative factors from the endothelium and increased mRNA iNOS expression. We also found that the incubation of HUVECs in the presence of propofol following ACE-Is pre-incubation caused weakness of the antifibrinolytic and pro-oxidative potential of propofol and this effect was similar after both ACE-Is. This observation suggests that the studied ACE-Is exerted protective effects against endothelial cell dysfunction caused by propofol, independently of hemodynamics.
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影响因子：
37.8
作者：
Ozkor MA;Murrow JR;Rahman AM;Kavtaradze N;Lin J;Manatunga A;Quyyumi AA
通讯作者：
Quyyumi AA
影响因子：
5.6
作者：
Lin, Ming-Chung;Lin, Chiou-Feng;Hsing, Chung-Hsi
通讯作者：
Hsing, Chung-Hsi
影响因子：
10.4
作者：
Aubert, H;Frère, C;Alessi, MC
通讯作者：
Alessi, MC
影响因子：
7.5
作者：
Gryglewski, RJ;Swies, J;Marcinkiewicz, E
通讯作者：
Marcinkiewicz, E
影响因子：
1.1
作者：
Chudy, Peter;Kotulicova, Daniela;Kubisz, Peter
通讯作者：
Kubisz, Peter