Bdellovibrio bacteriovorus inhibits Staphylococcus aureus biofilm formation and invasion into human epithelial cells.
Bdellovibrio bacteriovorus inhibits Staphylococcus aureus biofilm formation and invasion into human epithelial cells.
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DOI：
10.1038/srep03811
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发表时间：
2014-01-22
影响因子：
4.6
通讯作者：
Mitchell RJ
中科院分区：
文献类型：
--
作者：
Monnappa AK;Dwidar M;Seo JK;Hur JH;Mitchell RJ
噬菌蛭弧菌HD 100是一种捕食性细菌，攻击许多革兰氏阴性人类病原体。然而，该菌株的一个严重缺点是其对革兰氏阳性菌株（例如人类病原体金黄色葡萄球菌）无效。在这里，我们证明了由宿主非依赖性B. HIB能有效降解/抑制S.金黄色葡萄球菌生物膜，并降低其毒力。加入10%的HIB上清液可使S减少75%或更多。金黄色葡萄球菌生物膜形成以及预形成的生物膜的75%分散。LC-MS-MS分析鉴定了各种B。在一些实施方案中，所述蛋白酶包含上清液内的噬菌体蛋白酶，包括丝氨酸蛋白酶Bd 2269和Bd 2321。AEBSF试验证实丝氨酸蛋白酶在上清液中具有活性，并且它们影响S。金黄色葡萄球菌生物膜形成。上清液也具有轻微的DNA酶活性。此外，治疗强直性S。含有上清液的金黄色葡萄球菌使其侵袭MCF-10a上皮细胞的能力降低了5倍，但不影响MCF-10a的活力。总之，这项研究说明了B迄今为止未知的能力。噬菌菌体能够分散革兰氏阳性病原生物膜并减轻其毒力。
Bdellovibrio bacteriovorus HD100 is a predatory bacterium that attacks many Gram-negative human pathogens. A serious drawback of this strain, however, is its ineffectiveness against Gram-positive strains, such as the human pathogen Staphylococcus aureus. Here we demonstrate that the extracellular proteases produced by a host-independent B. bacteriovorus (HIB) effectively degrade/inhibit the formation of S. aureus biofilms and reduce its virulence. A 10% addition of HIB supernatant caused a 75% or greater reduction in S. aureus biofilm formation as well as 75% dispersal of pre-formed biofilms. LC-MS-MS analyses identified various B. bacteriovorus proteases within the supernatant, including the serine proteases Bd2269 and Bd2321. Tests with AEBSF confirmed that serine proteases were active in the supernatant and that they impacted S. aureus biofilm formation. The supernatant also possessed a slight DNAse activity. Furthermore, treatment of planktonic S. aureus with the supernatant diminished its ability to invade MCF-10a epithelial cells by 5-fold but did not affect the MCF-10a viability. In conclusion, this study illustrates the hitherto unknown ability of B. bacteriovorus to disperse Gram-positive pathogenic biofilms and mitigate their virulence.
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DOI：
10.3390/molecules14020586
发表时间：
2009-02-04
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Khan R;Islam B;Akram M;Shakil S;Ahmad A;Ali SM;Siddiqui M;Khan AU
通讯作者：
Khan AU
影响因子：
5.2
作者：
Gökçen A;Vilcinskas A;Wiesner J
通讯作者：
Wiesner J
影响因子：
14
作者：
Hetrick, Evan M.;Shin, Jae Ho;Paul, Heather S.;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
影响因子：
15.9
作者：
CHEUNG, AL;KRISHNAN, M;FISCHETTI, VA
通讯作者：
FISCHETTI, VA
影响因子：
3.8
作者：
Longhi, Catia;Scoarughi, Gian Luca;Selan, Laura
通讯作者：
Selan, Laura