Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquito Anopheles coluzzii.
Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquito Anopheles coluzzii.
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DOI：
10.1073/pnas.2112385119
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发表时间：
2022-06-07
影响因子：
11.1
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中科院分区：
文献类型：
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化学感觉系统在蚊子的整个生命周期中发挥着至关重要的作用，潜在的分子受体通常形成需要同源辅助受体的功能复合体。为了更好地了解疟疾媒介按蚊的化学感受途径，本研究利用基因编辑技术对嗜离子受体辅受体Acir76b进行了定位和功能鉴定。Acir76b在触角槽钉、体腔锥形感受器和其他附属嗅觉附件中均有表达。当Acir76b被灭活时，突变蚊子对胺的神经元反应发生了变化。除了嗅觉表型外，AcIr76b突变还表现出显著的交配和供血能力受损。我们的数据揭示了Acir76b在化学感觉通路中的离散作用，这表明电离通路是载体控制策略设计的目标。按蚊依靠其高度敏感的化学感受器来检测各种化学刺激，这些刺激驱动了寻找宿主和觅血行为，这是传播疟疾和其他疾病病原体所必需的。这一过程包括多种化学感受器和转导通路。我们使用先进的体内基因编辑和标记方法来定位和功能表征疟疾按蚊中的离子型共受体Acir76b，它影响嗅觉和味觉系统。Acir76b在雌性成虫触角节、腔锥形感受器、唇形感受器、口针感受器和眼轮感受器以及幼虫感觉器中有广泛的表达模式。Acir76b与Orco气味受体(OR)共定位于雌性触角和拉贝拉的一部分细胞中。与Orco和Ir8a不同，Oreco和Ir8a似乎对其各自的蚊子化学感觉神经元组的活动至关重要，而Acir76b−/−突变体保持了对成虫触须、唇部和幼虫感觉锥上的挥发胺的野生型外周反应。有趣的是，Acir76b−/−突变体在触角槽形感受器中对胺的反应显著增加，而腔锥型感受器对几种酸和胺的反应显著不足。在行为上，Acir76b突变体表现出显著的雌性特有的受精缺陷，尽管Acir76b−/−突变雌性可以定位、降落和探测人工血液宿主，但它们不能成功供血。综上所述，我们的发现揭示了Ir76b在按蚊嗅觉和味觉通路中的多维功能，直接影响这些蚊子的媒介能力。
Chemosensory systems play crucial roles across mosquito life cycles, with underlying molecular receptors often forming functional complexes that require cognate coreceptors. To better understand chemosensory pathways in the malaria vector mosquito Anopheles coluzzii, gene editing were used to localize and functionally characterize the ionotropic receptor coreceptor AcIr76b. Expression of AcIr76b was observed in antennal grooved pegs, coeloconic sensilla, and other accessory olfactory appendages. When AcIr76b was inactivated, mutant mosquitoes displayed altered neuronal responses to amines.. Beyond olfactory phenotypes, AcIr76b mutants display significantly impaired mating and blood feeding capabilities. Our data reveal discrete roles of AcIr76b across chemosensory pathways, suggesting ionotropic pathways as targets for the design of vector control strategies. Anopheline mosquitoes rely on their highly sensitive chemosensory apparatus to detect diverse chemical stimuli that drive the host-seeking and blood-feeding behaviors required to vector pathogens for malaria and other diseases. This process incorporates a variety of chemosensory receptors and transduction pathways. We used advanced in vivo gene-editing and -labeling approaches to localize and functionally characterize the ionotropic coreceptor AcIr76b in the malaria mosquito Anopheles coluzzii, where it impacts both olfactory and gustatory systems. AcIr76b has a broad expression pattern in female adult antennal grooved pegs, coeloconic sensilla, and T1 and T2 sensilla on the labellum, stylets, and tarsi, as well as the larval sensory peg. AcIr76b is colocalized with the Orco odorant receptor (OR) coreceptor in a subset of cells across the female antennae and labella. In contrast to Orco and Ir8a, chemosensory coreceptors that appear essential for the activity of their respective sets of chemosensory neurons in mosquitoes, AcIr76b−/− mutants maintain wild-type peripheral responses to volatile amines on the adult palps, labellum, and larval sensory cone. Interestingly, AcIr76b−/− mutants display significantly increased responses to amines in antennal grooved peg sensilla, while coeloconic sensilla reveal significant deficits in responses to several acids and amines. Behaviorally, AcIr76b mutants manifest significantly female-specific insemination deficits, and although AcIr76b−/− mutant females can locate, alight on, and probe artificial blood hosts, they are incapable of blood feeding successfully. Taken together, our findings reveal a multidimensional functionality of Ir76b in anopheline olfactory and gustatory pathways that directly impacts the vectorial capacity of these mosquitoes.
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影响因子：
16.2
作者：
Koh, Tong-Wey;He, Zhe;Gorur-Shandilya, Srinivas;Menuz, Karen;Larter, Nikki K.;Stewart, Shannon;Carlson, John R.
通讯作者：
Carlson, John R.
影响因子：
2.2
作者：
DENOTTER, CJ;BEHAN, M;MAES, FW
通讯作者：
MAES, FW
影响因子：
1.5
作者：
CHARLWOOD, JD;JONES, MDR
通讯作者：
JONES, MDR
影响因子：
9.8
作者：
He, Zhe;Luo, Yichen;Carlson, John R.
通讯作者：
Carlson, John R.
影响因子：
16.2
作者：
Jove, Veronica;Gong, Zhongyan;Hol, Felix J. H.;Zhao, Zhilei;Sorrells, Trevor R.;Carroll, Thomas S.;Prakash, Manu;McBride, Carolyn S.;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.