New methods for quantifying rapidity of action potential onset differentiate neuron types.
New methods for quantifying rapidity of action potential onset differentiate neuron types.
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DOI：
10.1371/journal.pone.0247242
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Lear KL
中科院分区：
文献类型：
--
作者：
Aldohbeyb AA;Vigh J;Lear KL
两种量化动作电位起搏速度的新方法相对标准偏差较低，且能较好地区分神经细胞类型。动作电位（ap）在大多数中枢哺乳动物神经元表现出急剧的动态。解释这种突然发生的主要观点各不相同。一些研究表明，突发性发作反映了钠离子通道的协同激活，而另一些研究则认为它们反映了轴突初始段的AP反向传播。然而，在这些研究中，AP的发病速度是主观定义的，通常使用相位图上任意值的斜率。因此，我们提出了更系统的方法，利用膜电位的二阶导数（）峰宽。本研究采用四种量化方法测量了4种不同皮层和海马神经元类型的AP速度：峰半最大值全宽倒数（IFWd2）、峰半最大值半宽度倒数（IHWd2）、相位图斜率和误差率法。IFWd2和IHWd2方法在同类型神经元中差异最小。此外，使用峰宽方法的AP快速性可以显著区分不同类型的神经元，表明AP快速性可以用于神经元类型的分类。使用IFWd2方法测量的AP速度能够区分所分析的所有四种神经元类型。因此，峰宽方法为研究影响AP发病动力学的机制提供了另一种灵敏的工具。
Two new methods for quantifying the rapidity of action potential onset have lower relative standard deviations and better distinguish neuron cell types than current methods. Action potentials (APs) in most central mammalian neurons exhibit sharp onset dynamics. The main views explaining such an abrupt onset differ. Some studies suggest sharp onsets reflect cooperative sodium channels activation, while others suggest they reflect AP backpropagation from the axon initial segment. However, AP onset rapidity is defined subjectively in these studies, often using the slope at an arbitrary value on the phase plot. Thus, we proposed more systematic methods using the membrane potential’s second-time derivative () peak width. Here, the AP rapidity was measured for four different cortical and hippocampal neuron types using four quantification methods: the inverse of full-width at the half maximum of the peak (IFWd2), the inverse of half-width at the half maximum of the peak (IHWd2), the phase plot slope, and the error ratio method. The IFWd2 and IHWd2 methods show the smallest variation among neurons of the same type. Furthermore, the AP rapidity, using the peak width methods, significantly differentiates between different types of neurons, indicating that AP rapidity can be used to classify neuron types. The AP rapidity measured using the IFWd2 method was able to differentiate between all four neuron types analyzed. Therefore, the peak width methods provide another sensitive tool to investigate the mechanisms impacting the AP onset dynamics.
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