16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers.
16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers.
复制标题
基于16S rRNA基因的微生物组分析鉴定出增加马铃薯块茎储存时间的候选菌株。
DOI：
10.1038/s41598-021-82181-9
复制
发表时间：
2021-02-04
影响因子：
4.6
通讯作者：
Mitter B
中科院分区：
文献类型：
--
作者：
Buchholz F;Junker R;Samad A;Antonielli L;Sarić N;Kostić T;Sessitsch A;Mitter B
在过去，已经详细研究了马铃薯植物微生物群和根际，以改善植物生长和适应性。然而，对马铃薯块茎微生物组及其在储存稳定性中的作用知之甚少。马铃薯的储存稳定性取决于基因型和储存条件，但块茎生长的土壤也可能发挥作用。为了了解采后马铃薯微生物群的生态和功能作用，我们在五种土壤类型中种植了四种马铃薯品种，并对其进行监测，直到块茎开始发芽。在储存期间，通过16S rRNA基因扩增子的下一代测序来分析块茎的细菌群落。马铃薯块茎的发芽行为表现出土壤依赖性差异。统计分析表明，与块茎相关的细菌群落从收获到休眠打破的强烈转变。通过结合指示种分析和相关矩阵，我们预测了细菌群落成员和块茎发芽行为之间的关联。在此基础上，我们鉴定了黄杆菌属菌株，其能够通过抑制马铃薯芽的生长来影响发芽行为。
In the past, the potato plant microbiota and rhizosphere have been studied in detail to improve plant growth and fitness. However, less is known about the postharvest potato tuber microbiome and its role in storage stability. The storage stability of potatoes depends on genotype and storage conditions, but the soil in which tubers were grown could also play a role. To understand the ecology and functional role of the postharvest potato microbiota, we planted four potato varieties in five soil types and monitored them until the tubers started sprouting. During storage, the bacterial community of tubers was analysed by next-generation sequencing of the 16S rRNA gene amplicons. The potato tubers exhibited soil-dependent differences in sprouting behaviour. The statistical analysis revealed a strong shift of the tuber-associated bacterial community from harvest to dormancy break. By combining indicator species analysis and a correlation matrix, we predicted associations between members of the bacterial community and tuber sprouting behaviour. Based on this, we identified Flavobacterium sp. isolates, which were able to influence sprouting behaviour by inhibiting potato bud outgrowth.
登录
查看更多内容
一种新的方法，通过将开花时的有益细菌引入后代种子来改变植物微生物和特征。
影响因子：
5.2
作者：
Mitter B;Pfaffenbichler N;Flavell R;Compant S;Antonielli L;Petric A;Berninger T;Naveed M;Sheibani-Tezerji R;von Maltzahn G;Sessitsch A
通讯作者：
Sessitsch A
影响因子：
10.7
作者：
Campisano, Andrea;Ometto, Lino;Rota-Stabelli, Omar
通讯作者：
Rota-Stabelli, Omar
影响因子：
5.2
作者：
Guyer A;De Vrieze M;Bönisch D;Gloor R;Musa T;Bodenhausen N;Bailly A;Weisskopf L
通讯作者：
Weisskopf L
影响因子：
3.7
作者：
Buchholz, Franziska;Antonielli, Livio;Mitter, Birgit
通讯作者：
Mitter, Birgit
影响因子：
14.9
作者：
CORPET, F
通讯作者：
CORPET, F