Gamma irradiation and microemulsion assisted synthesis of monodisperse flower-like platinum-gold nanoparticles/reduced graphene oxide nanocomposites for ultrasensitive detection of carcinoembryonic antigen
Gamma irradiation and microemulsion assisted synthesis of monodisperse flower-like platinum-gold nanoparticles/reduced graphene oxide nanocomposites for ultrasensitive detection of carcinoembryonic antigen
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伽马射线照射和微乳液辅助合成单分散花状铂金纳米颗粒/还原氧化石墨烯纳米复合材料用于超灵敏检测癌胚抗原
DOI：
10.1016/j.snb.2019.02.026
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发表时间：
2019-05
期刊：
影响因子：
--
通讯作者：
Xingxiang Zhang
中科院分区：
文献类型：
--
作者：
Xiaofan Li;Liangsen Liu;Zhiwei Xu;Wei Wang;Jie Shi;Liyan Liu;Miaolei Jing;Fengyan Li;Xingxiang Zhang
采用微乳液辅助γ射线辐照法制备了铂金（PtAu）/还原氧化石墨烯（rGO）纳米复合材料，PtAu纳米颗粒呈单分散的花状，粒径小于15 nm。γ辐照控制了氧化石墨烯和PtAu纳米粒子的还原，微乳液赋予PtAu纳米粒子多孔单分散的花状结构，抑制了纳米粒子的团聚。PtAu纳米颗粒的尺寸受到氧化石墨烯的限制效应和γ辐照的碎裂能力的限制。基于上述方法制备的rGO负载PtAu合金纳米粒子，构建了一种超灵敏、免标记的免疫传感器，用于检测癌胚抗原（CEA）。该花状PtAu/rGO纳米复合材料具有比其他纳米复合材料更高的法拉第电流，不仅在纳米尺度上提高了电极的电催化活性，增加了电极的表面积，而且为方便的抗体偶联提供了稳定的生物相容性基质，从而提供了一个高效的免疫分析平台。该免疫传感器具有良好的检测性能，检测限为7 fg·mL− 1，动态范围为10 fg·mL− 1 ~ 1 × 108 fg·mL−1。该免疫传感器对抗坏血酸、牛血清白蛋白、L-色氨酸和甲胎蛋白等干扰物具有良好的特异性。本工作的贡献在于研究了γ射线辐照和微乳液在形成花状PtAu NPs/rGO纳米颗粒中的协同作用，并将其应用于免疫传感器的制备。鉴于自组装纳米粒子的多功能性，该策略在免疫传感器和其他生物器件的制备中具有很大的应用前景。
The platinum-gold (PtAu)/reduced graphene oxide (rGO) nanocomposites were obtained via the gamma irradiation assisted by microemulsion while PtAu NPs showed the monodispersed flower-like shape with small-sized (below 15 nm). Gamma irradiation controlled the reduction of graphene oxide and PtAu NPs meanwhile the microemulsion endowed PtAu NPs with porous monodisperse flower-like structure and inhibited the agglomeration of nanoparticles. The size of PtAu NPs was limited by confinement effects of graphene oxide and fragmentation ability of gamma irradiation. An ultra-sensitive, label-free immunosensor was developed on the base of above rGO supported PtAu alloy nanoparticles for the determination of carcinoma embryonic antigen (CEA). The flower-like PtAu/rGO nanocomposites had a higher faradaic current than other nanocomposites, and the obtained PtAu NPs/rGO nanocomposites not only improve the electrocatalytic activity and increase the electrode surface area in nanoscale dimension, but also provide a stable and biocompatible matrix for the convenient conjugation of antibody, thus providing a high-efficiency immunoassay platform. The developed immunosensor showed excellent performance for the detection of CEA with a low detection limit of 7 fg·mL−1and a wide dynamic range from 10 fg·mL−1to 1 × 108fg·mL−1. In addition, the immunosensor system showed high specificity with the interference of ascorbic acid, bovine serum albumin, L-tryptophan and alpha fetal protein. The contribution of this work lies in the research of the synergistic effect of gamma irradiation and microemulsion in the formation of flower-like PtAu NPs/rGO nanoparticles applied in preparation of immunosensor. In view of the versatility of self-assembled nanoparticles, this strategy has great application prospects in fabrication of immunosensors and other biological devices.
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影响因子：
--
作者：
E. Katz;I. Willner
通讯作者：
E. Katz;I. Willner
DOI：
10.1016/j.colsurfa.2008.11.044
发表时间：
2009-04
期刊：
Colloids and Surfaces A: Physicochemical and Engineering Aspects
影响因子：
--
作者：
A. Pal;Sunil Shah;S. Belochapkine;D. Tanner;E. Magner;S. Devi
通讯作者：
A. Pal;Sunil Shah;S. Belochapkine;D. Tanner;E. Magner;S. Devi
影响因子：
12.6
作者：
Jing Wu;Junlin He;Chengli Zhang;Jun Chen;Y. Niu;Qiqi Yuan;Chao Yu
通讯作者：
Jing Wu;Junlin He;Chengli Zhang;Jun Chen;Y. Niu;Qiqi Yuan;Chao Yu
影响因子：
3.5
作者：
A. Guinier
通讯作者：
A. Guinier
影响因子：
3.7
作者：
Ranguwar Rajendra;Parnika Bhatia;A. Justin;Shilpy Sharma;N. Ballav
通讯作者：
Ranguwar Rajendra;Parnika Bhatia;A. Justin;Shilpy Sharma;N. Ballav