Atomic Resolution Structures of Human Bufaviruses Determined by Cryo-Electron Microscopy.
Atomic Resolution Structures of Human Bufaviruses Determined by Cryo-Electron Microscopy.
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DOI：
10.3390/v10010022
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发表时间：
2018-01-04
期刊：
影响因子：
--
通讯作者：
Agbandje-McKenna M
中科院分区：
文献类型：
--
作者：
Ilyas M;Mietzsch M;Kailasan S;Väisänen E;Luo M;Chipman P;Smith JK;Kurian J;Sousa D;McKenna R;Söderlund-Venermo M;Agbandje-McKenna M
Bufavirus 1 株 (BuV1) 是细小病毒科原细小病毒属的成员，首次从布基纳法索急性腹泻儿童的粪便样本中分离出来。自这一初步发现以来，包括芬兰、荷兰和不丹在内的多个国家已在表现出类似症状的儿科患者中分离出 BuV。为了表征，目前已知的基因型 BuV1、BuV2 和 BuV3 的病毒样颗粒的结构已通过冷冻电子显微镜和图像重建分别确定为 2.84、3.79 和 3.25 Å。 BuV 的氨基酸序列有 65-73% 相同，保留了细小病毒的主要病毒蛋白 VP2、结构和一般衣壳表面特征。这些包括核心 β-桶 (βB-βI)、α-螺旋 A 以及插入 VP2 中这些元件之间的大表面环。衣壳在二十面体2倍和5倍轴周围含有凹陷，并且在3倍轴周围具有三个分离的突起。 BuV 与现有细小病毒结构之间的结构比较揭示了衣壳表面变化和衣壳 3 倍突起，这些突起与动物原细小病毒的单风车排列不同。这些结构提供了一个平台来开始对这些潜在致病病毒进行分子表征。
Bufavirus strain 1 (BuV1), a member of the Protoparvovirus genus of the Parvoviridae, was first isolated from fecal samples of children with acute diarrhea in Burkina Faso. Since this initial discovery, BuVs have been isolated in several countries, including Finland, the Netherlands, and Bhutan, in pediatric patients exhibiting similar symptoms. Towards their characterization, the structures of virus-like particles of BuV1, BuV2, and BuV3, the current known genotypes, have been determined by cryo-electron microscopy and image reconstruction to 2.84, 3.79, and 3.25 Å, respectively. The BuVs, 65–73% identical in amino acid sequence, conserve the major viral protein, VP2, structure and general capsid surface features of parvoviruses. These include a core β-barrel (βB-βI), α-helix A, and large surface loops inserted between these elements in VP2. The capsid contains depressions at the icosahedral 2-fold and around the 5-fold axes, and has three separated protrusions surrounding the 3-fold axes. Structure comparison among the BuVs and to available parvovirus structures revealed capsid surface variations and capsid 3-fold protrusions that depart from the single pinwheel arrangement of the animal protoparvoviruses. These structures provide a platform to begin the molecular characterization of these potentially pathogenic viruses.
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DOI：
10.1086/652416
发表时间：
2010-06-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Kapoor A;Simmonds P;Slikas E;Li L;Bodhidatta L;Sethabutr O;Triki H;Bahri O;Oderinde BS;Baba MM;Bukbuk DN;Besser J;Bartkus J;Delwart E
通讯作者：
Delwart E
DOI：
10.1073/pnas.93.15.7502
发表时间：
1996-07-23
影响因子：
11.1
作者：
Chipman, PR;AgbandjeMcKenna, M;Rossmann, MG
通讯作者：
Rossmann, MG
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
5.4
作者：
Kailasan, Shweta;Garrison, Jamie;Agbandje-McKenna, Mavis
通讯作者：
Agbandje-McKenna, Mavis
DOI：
10.1073/pnas.0504666102
发表时间：
2005-09-06
影响因子：
11.1
作者：
Allander, T;Tammi, MT;Andersson, B
通讯作者：
Andersson, B