Rat adipose tissue-derived stem cells transplantation attenuates cardiac dysfunction post infarction and biopolymers enhance cell retention.
Rat adipose tissue-derived stem cells transplantation attenuates cardiac dysfunction post infarction and biopolymers enhance cell retention.
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DOI：
10.1371/journal.pone.0012077
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发表时间：
2010-08-10
期刊：
影响因子：
3.7
通讯作者：
Krieger JE
中科院分区：
文献类型：
--
作者：
Danoviz ME;Nakamuta JS;Marques FL;dos Santos L;Alvarenga EC;dos Santos AA;Antonio EL;Schettert IT;Tucci PJ;Krieger JE
心脏细胞移植受到低细胞保留和差移植物活力的影响。在此，在心肌梗死（MI）大鼠模型中评价了联合注射脂肪组织来源的干细胞（ASC）和生物聚合物对细胞心脏滞留、心室形态和性能的影响。 MI后24小时，使用纤维蛋白a、胶原（ASC/C）或培养基（ASC/M）作为载体，注射从同基因刘易斯大鼠分离的99 mTc标记的ASC（1×106个细胞），24小时后通过收获器官的γ发射计数评估细胞体分布。ASC/F和ASC/C组在心肌中保留的细胞显著多于ASC/M组（分别为13.8±2.0和26.8±2.4%对4.8± 0.7%）。然后，在MI细胞注射后4周评价形态测量和直接心脏功能参数。与未处理组（NT）和对照组（仅培养基、纤维蛋白或胶原蛋白）相比，所有ASC处理组的左心室（LV）周长和备用心肌间质胶原蛋白百分比均显著减弱。药理学应激下的直接血流动力学评估显示，无论用于递送ASC的载体如何，ASC治疗组的每搏输出量（SV）和左心室舒张末期压均保持不变。心搏功（SW）是心脏功能的一个整体指数，ASC/M改善，而生物聚合物与ASC共注射时则正常化。在血流动力学应激下，观察到心脏ASC保留和SV保留与MI后SW改善之间呈正相关。我们提供了直接的证据表明，心肌内注射ASC减轻了大鼠MI后的负性心脏重塑并保留了心室功能，并且这些有益作用可以通过将ASC与生物聚合物共同注射而进一步增强。
Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI). 99mTc-labeled ASCs (1×106 cells) isolated from isogenic Lewis rats were injected 24 hours post-MI using fibrin a, collagen (ASC/C), or culture medium (ASC/M) as vehicle, and cell body distribution was assessed 24 hours later by γ-emission counting of harvested organs. ASC/F and ASC/C groups retained significantly more cells in the myocardium than ASC/M (13.8±2.0 and 26.8±2.4% vs. 4.8±0.7%, respectively). Then, morphometric and direct cardiac functional parameters were evaluated 4 weeks post-MI cell injection. Left ventricle (LV) perimeter and percentage of interstitial collagen in the spare myocardium were significantly attenuated in all ASC-treated groups compared to the non-treated (NT) and control groups (culture medium, fibrin, or collagen alone). Direct hemodynamic assessment under pharmacological stress showed that stroke volume (SV) and left ventricle end-diastolic pressure were preserved in ASC-treated groups regardless of the vehicle used to deliver ASCs. Stroke work (SW), a global index of cardiac function, improved in ASC/M while it normalized when biopolymers were co-injected with ASCs. A positive correlation was observed between cardiac ASCs retention and preservation of SV and improvement in SW post-MI under hemodynamic stress. We provided direct evidence that intramyocardial injection of ASCs mitigates the negative cardiac remodeling and preserves ventricular function post-MI in rats and these beneficial effects can be further enhanced by administrating co-injection of ASCs with biopolymers.
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影响因子：
2.9
作者：
Blande, Ivan Souza;Bassaneze, Vinicius;Krieger, Jose Eduardo
通讯作者：
Krieger, Jose Eduardo
影响因子：
18.2
作者：
Mazo, Manuel;Planat-Benard, Valerie;Prosper, Felipe
通讯作者：
Prosper, Felipe
影响因子：
4
作者：
Bassaneze, Vinicius;Barauna, Valerio Garrone;Krieger, Jose Eduardo
通讯作者：
Krieger, Jose Eduardo
DOI：
10.1016/j.bbrc.2007.01.045
发表时间：
2007-03-16
影响因子：
3.1
作者：
Iso, Yoshitaka;Spees, Jeffrey L.;Prockop, Darwin J.
通讯作者：
Prockop, Darwin J.
影响因子：
37.8
作者：
LITWIN, SE;KATZ, SE;DOUGLAS, PS
通讯作者：
DOUGLAS, PS