Dietary n-6 polyunsaturated fatty acid deprivation increases docosahexaenoic acid metabolism in rat brain.
Dietary n-6 polyunsaturated fatty acid deprivation increases docosahexaenoic acid metabolism in rat brain.
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DOI：
10.1111/j.1471-4159.2011.07597.x
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发表时间：
2012-03
影响因子：
4.7
通讯作者：
Rapoport SI
中科院分区：
文献类型：
--
作者：
Igarashi M;Kim HW;Chang L;Ma K;Rapoport SI
Dietary n-6 polyunsaturated fatty acid (PUFA) deprivation in rodents reduces brain arachidonic acid (20:4n-6) concentration and 20:4n-6-preferring cytosolic phospholipase A2 (cPLA2-IVA) and cyclooxygenase (COX)-2 expression, while increasing brain docosahexaenoic acid (DHA, 22:6n-3) concentration and DHA-selective Ca2+-independent iPLA2-VIA expression. We hypothesized that these changes are accompanied by upregulated brain DHA metabolic rates. Using a fatty acid model, brain DHA concentrations and kinetics were measured in unanesthetized male rats fed, for 15 weeks post-weaning, an n-6 PUFA “adequate” (31.4 wt% linoleic acid) or “deficient” (2.7 wt% linoleic acid) diet, each lacking 20:4n-6 and DHA. [1-14C]DHA was infused intravenously, arterial blood was sampled, and the brain was microwaved at 5 min and analyzed. Rats fed the n-6 PUFA deficient compared with adequate diet had significantly reduced n-6 PUFA concentrations in brain phospholipids but increased eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acidn-3 (DPAn-3, 22:5n-3) and DHA (by 9.4%) concentrations, particularly in ethanolamine glycerophospholipid. Incorporation rates of unesterified DHA from plasma, which represent DHA metabolic loss from brain, were increased 45% in brain phospholipids, as was DHA turnover. Increased DHA metabolism following dietary n-6 PUFA deprivation may increase brain concentrations of antiinflammatory DHA metabolites, which with a reduced brain n-6 PUFA content, likely promote neuroprotection. (199 words)
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影响因子：
6.5
作者：
Gao, Fei;Kiesewetter, Dale;Igarashi, Miki
通讯作者：
Igarashi, Miki
影响因子：
4.2
作者：
BOURRE, JM;DURAND, G;YOUYOU, A
通讯作者：
YOUYOU, A
DOI：
10.1016/j.bbalip.2011.05.008
发表时间：
2011-07-01
影响因子：
4.8
作者：
Gao, Fei;Kim, Hyung-Wook;Rapoport, Stanley I.
通讯作者：
Rapoport, Stanley I.
影响因子：
4.2
作者：
BOURRE, JM;FRANCOIS, M;DURAND, G
通讯作者：
DURAND, G
影响因子：
6.3
作者：
Basselin, Mireille;Ramadan, Epolia;Rapoport, Stanley I.
通讯作者：
Rapoport, Stanley I.