Multi-channel hyperspectral fluorescence detection excited by coupled plasmon-waveguide resonance.
Multi-channel hyperspectral fluorescence detection excited by coupled plasmon-waveguide resonance.
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DOI：
10.3390/s131013892
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发表时间：
2013-10-14
期刊：
影响因子：
--
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Du C;Liu L;Zhang L;Guo J;Guo J;Ma H;He Y
We propose in this paper a biosensor scheme based on coupled plasmon-waveguide resonance (CPWR) excited fluorescence spectroscopy. A symmetrical structure that offers higher surface electric field strengths, longer surface propagation lengths and depths is developed to support guided waveguide modes for the efficient excitation of fluorescence. The optimal parameters for the sensor films are theoretically and experimentally investigated, leading to a detection limit of 0.1 nM (for a Cy5 solution). Multiplex analysis possible with the fluorescence detection is further advanced by employing the hyperspectral fluorescence technique to record the full spectra for every pixel on the sample plane. We demonstrate experimentally that highly overlapping fluorescence (Cy5 and Dylight680) can be distinguished and ratios of different emission sources can be determined accurately. This biosensor shows great potential for multiplex detections of fluorescence analytes.
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影响因子：
15
作者：
Leevy, W. Matthew;Gammon, Seth T.;Smith, Bradley D.
通讯作者：
Smith, Bradley D.
DOI：
10.1016/s0927-7757(99)00550-6
发表时间：
2000-10-10
影响因子：
5.2
作者：
Liebermann, T;Knoll, W
通讯作者：
Knoll, W
DOI：
10.1073/pnas.96.4.1375
发表时间：
1999-02-16
影响因子：
11.1
作者：
Winkler, T;Kettling, U;Eigen, M
通讯作者：
Eigen, M
针对多色微阵列分析而优化的准共焦、多通道并行扫描高光谱荧光成像方法
影响因子：
7.4
作者：
Liu, Zhiyi;Ma, Suihua;He, Yonghong
通讯作者：
He, Yonghong
影响因子：
7.4
作者：
Deng, Wei;Drozdowicz-Tomsia, Krystyna;Goldys, Ewa M.
通讯作者：
Goldys, Ewa M.