Improvement of the diagnostic sensitivity of scrub typhus using a mixture of recombinant antigens derived from Orientia tsutsugamushi serotypes.
Improvement of the diagnostic sensitivity of scrub typhus using a mixture of recombinant antigens derived from Orientia tsutsugamushi serotypes.
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DOI：
10.3346/jkms.2013.28.5.672
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发表时间：
2013-05
影响因子：
4.5
通讯作者：
Kim YW
中科院分区：
文献类型：
--
作者：
Kim YJ;Yeo SJ;Park SJ;Woo YJ;Kim MW;Kim SH;Chang IA;Jeon SH;Park BJ;Song GJ;Lee MG;Kim IS;Kim YW
恙虫病的诊断是困难的，因为它的症状与其他急性发热性疾病，如钩端螺旋体病、鼠斑疹伤寒和其他病毒性出血热非常相似。为了区分恙虫病与其他急性发热性疾病，快速可靠的血清学诊断至关重要。我们从恙虫病东方体的不同血清型中开发了一种嵌合重组抗原cr56和另外两种重组抗原r21和kr56。使用酶联免疫吸附试验（ELISA）和点印迹分析检测患者血清样本中的恙虫病恙虫体抗体。与传统的免疫荧光法（IFA）一样，当使用这三种重组抗原的混合物时，在ELISA和dot blotting中，重组抗原对IgM和IgG的敏感性和特异性均提高了98%。此外，在快速诊断试验（RDT）中，使用三种抗原和金偶联抗体的混合物检测IgM和IgG抗体的灵敏度和特异性均为99%。本研究结果表明，在ELISA或RDT中使用这些重组抗原蛋白的混合物可作为恙虫病的诊断试验。
Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub typhus from other acute febrile diseases, a rapid and reliable serological diagnosis is important. We have developed a chimeric recombinant antigen cr56 and two other recombinant antigens, r21 and kr56, from various serotypes of Orientia tsutsugamushi. They were tested for the detection of antibodies against O. tsutsugamushi in the patient's serum samples using enzyme-linked immunosorbent assay (ELISA) and dot-blot analyses. As of conventional immunofluorescence assay (IFA), when the mixture of these three recombinant antigens was used, both sensitivity and specificity of the recombinant antigens were increased up to 98% in IgM and IgG at ELISA and dot blotting. Additionally, both sensitivity and specificity by detection of IgM and IgG antibodies at rapid diagnostic test (RDT), using the mixture of three antigens and gold conjugated antibodies, were 99%. Our results suggest the use of mixture of these recombinant antigen proteins in ELISA or RDT is suitable as a diagnostic test for scrub typhus.
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DOI：
10.4269/ajtmh.1983.32.1101
发表时间：
1983-01-01
影响因子：
3.3
作者：
BROWN, GW;SHIRAI, A;GROVES, MG
通讯作者：
GROVES, MG
影响因子：
2.1
作者：
Enatsu, T;Urakami, HL;Tamura, A
通讯作者：
Tamura, A
DOI：
10.4269/ajtmh.2003.69.60
发表时间：
2003-07-01
影响因子：
3.3
作者：
Jiang, J;Marienau, KJ;Richards, AL
通讯作者：
Richards, AL
影响因子：
3.1
作者：
Choi, MS;Seong, SY;Kim, IS
通讯作者：
Kim, IS
影响因子：
6.4
作者：
RAPMUND, G
通讯作者：
RAPMUND, G