Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer.
Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer.
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DOI：
10.1186/s12885-018-4772-0
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发表时间：
2018-09-18
期刊：
影响因子：
3.8
通讯作者：
Sozzi G
中科院分区：
文献类型：
--
作者：
Andriani F;Landoni E;Mensah M;Facchinetti F;Miceli R;Tagliabue E;Giussani M;Callari M;De Cecco L;Colombo MP;Roz L;Pastorino U;Sozzi G
癌细胞与周围微环境之间的相互作用是癌症进展的关键决定因素。在此过程中，肿瘤细胞和癌症相关成纤维细胞 (CAF) 之间的双向通讯调节细胞外基质 (ECM) 沉积和重塑。这一动态过程的结果是，可溶性 ECM 蛋白可以释放到血液中，并可能代表可用于癌症诊断的新型循环生物标志物。本研究的目的是测量肺癌患者和健康重度吸烟者对照血浆样本中三种循环 ECM 相关蛋白（COL11A1、COL10A1 和 SPARC）的水平，并测试此类测量是否具有诊断或预后价值。通过基因表达微阵列对从配对的 NSCLC 患者正常组织和癌症组织中分离出的肺成纤维细胞进行基因表达谱分析。研究血浆循环蛋白的候选者的优先顺序是基于癌症相关成纤维细胞中差异最大的表达基因。通过肺成纤维细胞条件培养基中的蛋白质印迹和血浆样品中的 ELISA 测定来评估可溶性 ECM 蛋白。对肺癌患者和健康重度吸烟者对照者的血浆样本进行了 COL11A1 和 COL10A1（各 n = 57）和 SPARC（各 n = 90）水平的检测。在患者中检测到较高的血浆 COL10A1 水平 (p≤≤0.001)，这一差异主要由女性引起 (p≤0.001)。未发现患者和对照组之间的 COL11A1 水平存在差异。血浆患者中的 SPARC 水平也高于对照组 (p<0.001)，在区分两组方面表现良好 (AUC=0.744)。血浆蛋白水平与临床病理特征或生存率之间没有观察到显着关联。在原发性肺癌患者的血浆中可以检测到与熟练的肿瘤-基质串扰相关的可溶性因子，并且可能代表一种有价值的补充诊断工具，用于区分肺癌患者与健康的重度吸烟者个体，如 SPARC 蛋白所示。本文的在线版本 (10.1186/s12885-018-4772-0) 包含补充材料，可供授权用户使用。
Interactions between cancer cells and the surrounding microenvironment are crucial determinants of cancer progression. During this process, bi-directional communication among tumor cells and cancer associated fibroblasts (CAF) regulate extracellular matrix (ECM) deposition and remodeling. As a result of this dynamic process, soluble ECM proteins can be released into the bloodstream and may represent novel circulating biomarkers useful for cancer diagnosis. The aim of the present study was to measure the levels of three circulating ECM related proteins (COL11A1, COL10A1 and SPARC) in plasma samples of lung cancer patients and in healthy heavy-smokers controls and test whether such measurements have diagnostic or prognostic value. Gene expression profiling of lung fibroblasts isolated from paired normal and cancer tissue of NSCLC patients was performed by gene expression microarrays. The prioritization of the candidates for the study of circulating proteins in plasma was based on the most differentially expressed genes in cancer associated fibroblasts. Soluble ECM proteins were assessed by western blot in the conditioned medium of lung fibroblasts and by ELISA assays in plasma samples. Plasma samples from lung cancer patients and healthy heavy-smokers controls were tested for levels of COL11A1 and COL10A1 (n = 57 each) and SPARC (n = 90 each). Higher plasma levels of COL10A1 were detected in patients (p ≤ 0.001), a difference that was driven specifically by females (p < 0.001). No difference in COL11A1 levels between patients and controls was found. SPARC levels were also higher in plasma patients than controls (p < 0.001) with good performance in discriminating the two groups (AUC = 0.744). No significant association was observed between plasma proteins levels and clinicopathological features or survival. Soluble factors related to proficient tumor-stroma cross-talk are detectable in plasma of primary lung cancer patients and may represent a valuable complementary diagnostic tool to discriminate lung cancer patients from healthy heavy-smokers individuals as shown for the SPARC protein. The online version of this article (10.1186/s12885-018-4772-0) contains supplementary material, which is available to authorized users.
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影响因子：
2
作者：
DURRLEMAN, S;SIMON, R
通讯作者：
SIMON, R
影响因子：
3.7
作者：
de Torres JP;Casanova C;Pinto-Plata V;Varo N;Restituto P;Cordoba-Lanus E;Baz-Dávila R;Aguirre-Jaime A;Celli BR
通讯作者：
Celli BR
影响因子：
8.8
作者：
Kuchnio, Anna;Moens, Stijn;Carmeliet, Peter
通讯作者：
Carmeliet, Peter
影响因子：
--
作者：
Hung JY;Yen MC;Jian SF;Wu CY;Chang WA;Liu KT;Hsu YL;Chong IW;Kuo PL
通讯作者：
Kuo PL
DOI：
10.12659/msm.898675
发表时间：
2016-07-21
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Bilfinger T;Keresztes R;Albano D;Nemesure B
通讯作者：
Nemesure B