Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings.
Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings.
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DOI：
10.1186/1471-2229-13-124
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发表时间：
2013-09-05
影响因子：
5.3
通讯作者：
Medina FJ
中科院分区：
文献类型：
--
作者：
Manzano AI;Larkin OJ;Dijkstra CE;Anthony P;Davey MR;Eaves L;Hill RJ;Herranz R;Medina FJ
细胞生长和细胞增殖在地球重力存在下密切相关，但在轨道航天器中存在的微重力条件下是解耦的。需要新技术在地球实验室中模拟微重力条件，在稳定的环境条件下进行长期实验，以进一步了解地外条件对生命物质生长、发育和健康的影响。我们研究了含有 CycB1-GUS 增殖标记或 DR5-GUS 生长素介导的生长标记的拟南芥转基因幼苗对超导螺线管磁体孔中抗磁悬浮的反应。作为对照，第二组幼苗暴露于强磁场，但不暴露于悬浮力。第三组暴露于强场和模拟超重力（2g）。测量每组幼苗的细胞增殖和细胞生长细胞学参数。核仁蛋白免疫检测用作细胞生长的标记。总的来说，数据表明，这两个基本细胞过程在根分生组织中是解耦的，就像在微重力下一样：细胞增殖增强，而细胞生长标记物减少。这些结果还证明了根尖中生长素信号的离域化，尽管幼苗整体的悬浮并不能阻止根细胞中平衡石的沉积。在我们的模型系统中，我们发现反磁悬浮导致的变化与真实的变化非常相似。在国际空间站 (ISS) 上] 或机械模拟微重力 [例如使用随机定位机（RPM）]。这些变化使分生组织细胞增殖与核糖体生物发生脱钩，并改变了生长素极性运输。
Cell growth and cell proliferation are intimately linked in the presence of Earth’s gravity, but are decoupled under the microgravity conditions present in orbiting spacecraft. New technologies to simulate microgravity conditions for long-duration experiments, with stable environmental conditions, in Earth-based laboratories are required to further our understanding of the effect of extraterrestrial conditions on the growth, development and health of living matter. We studied the response of transgenic seedlings of Arabidopsis thaliana, containing either the CycB1-GUS proliferation marker or the DR5-GUS auxin-mediated growth marker, to diamagnetic levitation in the bore of a superconducting solenoid magnet. As a control, a second set of seedlings were exposed to a strong magnetic field, but not to levitation forces. A third set was exposed to a strong field and simulated hypergravity (2 g). Cell proliferation and cell growth cytological parameters were measured for each set of seedlings. Nucleolin immunodetection was used as a marker of cell growth. Collectively, the data indicate that these two fundamental cellular processes are decoupled in root meristems, as in microgravity: cell proliferation was enhanced whereas cell growth markers were depleted. These results also demonstrated delocalisation of auxin signalling in the root tip despite the fact that levitation of the seedling as a whole does not prevent the sedimentation of statoliths in the root cells. In our model system, we found that diamagnetic levitation led to changes that are very similar to those caused by real- [e.g. on board the International Space Station (ISS)] or mechanically-simulated microgravity [e.g. using a Random Positioning Machine (RPM)]. These changes decoupled meristematic cell proliferation from ribosome biogenesis, and altered auxin polar transport.
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影响因子：
11.6
作者：
Casimiro, I;Marchant, A;Bennett, M
通讯作者：
Bennett, M
影响因子：
11.6
作者：
FERREIRA, PCG;HEMERLY, AS;INZE, D
通讯作者：
INZE, D
影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
2.3
作者：
González-Camacho, F;Medina, FJ
通讯作者：
Medina, FJ
影响因子：
5.7
作者：
Florez, Mercedes;Carbonell, Maria Victoria;Martinez, Elvira
通讯作者：
Martinez, Elvira