ICE1 of Poncirus trifoliata functions in cold tolerance by modulating polyamine levels through interacting with arginine decarboxylase.
ICE1 of Poncirus trifoliata functions in cold tolerance by modulating polyamine levels through interacting with arginine decarboxylase.
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DOI：
10.1093/jxb/erv138
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发表时间：
2015-06
影响因子：
6.9
通讯作者：
Liu JH
中科院分区：
文献类型：
--
作者：
Huang XS;Zhang Q;Zhu D;Fu X;Wang M;Zhang Q;Moriguchi T;Liu JH
三叶海棠ICE1(L.)英国皇家空军。在耐寒中发挥积极作用，并通过与精氨酸脱羧酶相互作用来调节多胺的合成。ICE1(Inducer Of CBF Expression 1)编码一种MYC样碱性螺旋-环-螺旋转录因子，作为冷反应的中央调节因子。在本研究中，我们从三叶橙[PonCirus trifoliata(L.)Raf.]。PtrICE1在低温、脱水和盐条件下表达上调，在低温条件下的诱导作用最强。PtrICE1定位于胞核，可与MYC识别序列结合。PtrICE1在烟草和柠檬中的异位表达增强了烟草和柠檬在低温或冰冻温度下对冷胁迫的耐性。酵母双杂交筛选表明，21个蛋白质属于PtrICE1相互作用组，其中PtADC(精氨酸脱羧酶)被证实为与PtrICE1相互作用的真实蛋白质。转基因株系中ADC基因的转录水平在正常生长条件下略有升高，但在低温条件下显著增加，这与游离多胺水平的变化一致。相比之下，在低温胁迫下，转基因品系中过氧化氢和超氧阴离子的积累明显减轻。在低温条件下，转基因植株具有较高的抗氧化物酶活性，如超氧化物歧化酶和过氧化氢酶。综上所述，这些结果表明PtrICE1在耐冷性方面发挥了积极的作用，这可能是由于通过与ADC基因的相互作用来调节多胺水平。
ICE1 of Poncirus trifoliata (L.) Raf. plays a positive role in cold tolerance and modulates polyamine synthesis by interacting with arginine decarboxylase. ICE1 (Inducer of CBF Expression 1) encodes a MYC-like basic helix–loop–helix transcription factor that acts as a central regulator of cold response. In this study, we elucidated the function and underlying mechanisms of PtrICE1 from trifoliate orange [Poncirus trifoliata (L.) Raf.]. PtrICE1 was upregulated by cold, dehydration, and salt, with the greatest induction under cold conditions. PtrICE1 was localized in the nucleus and could bind to a MYC-recognizing sequence. Ectopic expression of PtrICE1 in tobacco and lemon conferred enhanced tolerance to cold stresses at either chilling or freezing temperatures. Yeast two-hybrid screening revealed that 21 proteins belonged to the PtrICE1 interactome, in which PtADC (arginine decarboxylase) was confirmed as a bona fide protein interacting with PtrICE1. Transcript levels of ADC genes in the transgenic lines were slightly elevated under normal growth condition but substantially increased under cold conditions, consistent with changes in free polyamine levels. By contrast, accumulation of the reactive oxygen species, H2O2 and O2 –, was appreciably alleviated in the transgenic lines under cold stress. Higher activities of antioxidant enzymes, such as superoxide dismutase and catalase, were detected in the transgenic lines under cold conditions. Taken together, these results demonstrated that PtrICE1 plays a positive role in cold tolerance, which may be due to modulation of polyamine levels through interacting with the ADC gene.
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