Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.
Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.
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DOI：
10.1152/ajpendo.00236.2021
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发表时间：
2021-12-01
期刊：
影响因子：
--
通讯作者：
Davis TA
中科院分区：
文献类型：
--
作者：
Rudar M;Naberhuis JK;Suryawan A;Nguyen HV;Stoll B;Style CC;Verla MA;Olutoye OO;Burrin DG;Fiorotto ML;Davis TA
优化肠内营养早产儿可能有助于减轻宫外生长限制和不良的慢性健康结局。以前，我们在足月出生的新生猪中发现，与连续喂养相比，间歇性喂养可以促进瘦生长。目的是确定早产是否会影响身体成分、肌肉蛋白质合成和肌核增生对喂养方式的反应。在早产后，猪被喂食等量的配方奶，间歇给药（INT, n = 30）或连续给药（CONT, n = 14） 21天。用双能x线吸收仪（DXA）测量体成分，用形态测定法、肌核增生和卫星细胞丰度评估肌肉生长。在INT猪吸收后（INT- pa）和餐后（INT- pp）状态和CONT猪中测定组织合成代谢信号和部分蛋白质合成率。INT和CONT猪的体增重和成分没有差异。INT-PP组背最长肌蛋白质合成比INT-PA组高34% (P < 0.05)，而INT-PP组与CONT组之间差异不显著。LD中4EBP1和S6K1的磷酸化以及eIF4E·eIF4G丰度与LD蛋白合成的变化平行。LD的卫星细胞丰度、肌核增生和纤维截面积在两组间无差异。这些结果表明，与足月出生的猪不同，间歇性大剂量喂养并不比连续喂养更能促进早产儿的瘦肉生长。早产减弱了骨骼肌对胰岛素和氨基酸周期性激增的反应能力，并在出生后早期间歇性喂养。新的和值得注意的宫外生长限制经常发生在早产儿，但可以通过优化肠内喂养策略来减轻。我们发现，与连续喂养相比，间歇性大剂量喂养不会增加早产儿骨骼肌蛋白质合成、肌核增生或瘦肉生长。与之前在足月出生的猪中观察到的情况相比，这种肌肉对间歇性大剂量喂养的合成代谢反应减弱，可能导致许多早产儿在成年后表现出瘦质量缺陷。
Optimizing enteral nutrition for premature infants may help mitigate extrauterine growth restriction and adverse chronic health outcomes. Previously, we showed in neonatal pigs born at term that lean growth is enhanced by intermittent bolus compared with continuous feeding. The objective was to determine if prematurity impacts how body composition, muscle protein synthesis, and myonuclear accretion respond to feeding modality. Following preterm delivery, pigs were fed equivalent amounts of formula delivered either as intermittent boluses (INT; n = 30) or continuously (CONT; n = 14) for 21 days. Body composition was measured by dual-energy X-ray absorptiometry (DXA) and muscle growth was assessed by morphometry, myonuclear accretion, and satellite cell abundance. Tissue anabolic signaling and fractional protein synthesis rates were determined in INT pigs in postabsorptive (INT-PA) and postprandial (INT-PP) states and in CONT pigs. Body weight gain and composition did not differ between INT and CONT pigs. Longissimus dorsi (LD) protein synthesis was 34% greater in INT-PP than INT-PA pigs (P < 0.05) but was not different between INT-PP and CONT pigs. Phosphorylation of 4EBP1 and S6K1 and eIF4E·eIF4G abundance in LD paralleled changes in LD protein synthesis. Satellite cell abundance, myonuclear accretion, and fiber cross-sectional area in LD did not differ between groups. These results suggest that, unlike pigs born at term, intermittent bolus feeding does not enhance lean growth more than continuous feeding in pigs born preterm. Premature birth attenuates the capacity of skeletal muscle to respond to cyclical surges in insulin and amino acids with intermittent feeding in early postnatal life. NEW & NOTEWORTHY Extrauterine growth restriction often occurs in premature infants but may be mitigated by optimizing enteral feeding strategies. We show that intermittent bolus feeding does not increase skeletal muscle protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in preterm pigs. This attenuated anabolic response of muscle to intermittent bolus feeding, compared with previous observations in pigs born at term, may contribute to deficits in lean mass that many premature infants exhibit into adulthood.
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DOI：
10.1242/dev.167197
发表时间：
2018-10-25
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Bachman JF;Klose A;Liu W;Paris ND;Blanc RS;Schmalz M;Knapp E;Chakkalakal JV
通讯作者：
Chakkalakal JV
影响因子：
3.6
作者：
El-Kadi, Samer W.;Gazzaneo, Maria C.;Suryawan, Agus;Orellana, Renan A.;Torrazza, Roberto Murgas;Srivastava, Neeraj;Kimball, Scot R.;Nguyen, Hanh V.;Fiorotto, Marta L.;Davis, Teresa A.
通讯作者：
Davis, Teresa A.
DOI：
10.1152/ajpendo.00516.2011
发表时间：
2012-03-01
影响因子：
5.1
作者：
El-Kadi, Samer W.;Suryawan, Agus;Davis, Teresa A.
通讯作者：
Davis, Teresa A.
DOI：
10.1152/ajpendo.00520.2015
发表时间：
2016-06-01
影响因子：
5.1
作者：
Kao, Michelle;Columbus, Daniel A.;Davis, Teresa A.
通讯作者：
Davis, Teresa A.
DOI：
10.1152/ajpendo.00510.2004
发表时间：
2005-05-01
影响因子：
5.1
作者：
Escobar, J;Frank, JW;Davis, TA
通讯作者：
Davis, TA