Viscera and muscle protein synthesis in neonatal pigs is increased more by intermittent bolus than by continuous feeding.
Viscera and muscle protein synthesis in neonatal pigs is increased more by intermittent bolus than by continuous feeding.
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DOI：
10.1038/pr.2013.89
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发表时间：
2013-08
影响因子：
3.6
通讯作者：
Davis, Teresa A.
中科院分区：
文献类型：
--
作者：
El-Kadi, Samer W.;Gazzaneo, Maria C.;Suryawan, Agus;Orellana, Renan A.;Torrazza, Roberto Murgas;Srivastava, Neeraj;Kimball, Scot R.;Nguyen, Hanh V.;Fiorotto, Marta L.;Davis, Teresa A.
连续和间歇性团口胃喂养是用于不能耐受正常喂养的婴儿的策略。为了确定喂养方式对不同组织中蛋白质合成的影响，新生猪通过口胃管以每4小时间歇推注喂养或连续输注的方式接受平衡配方，或禁食过夜。与禁食相比，蛋白质合成在腓肠肌，咬肌，比目鱼肌，左心室，肝脏，胰腺，空肠，肾脏增加在丸和连续喂养的猪，但最大的增加发生在丸餐后。肝硬化复合体（TSC 2），富含脯氨酸的AKT底物40 kDa（PRAS 40），真核起始因子（eIF）4E结合蛋白（4EBP 1）和rp S6激酶1（S6 K1）磷酸化在所有组织和核糖体蛋白S4在肝多聚核糖体中的比例增强后90分钟，餐前或连续喂养期间，但不立即。真核细胞延伸因子2（eEF 2）和eIF 2 α磷酸化不受喂养的影响。这些结果表明，间歇性大剂量喂养增加蛋白质合成的不同纤维类型的肌肉和内脏组织在更大程度上比连续喂养刺激翻译启动。
Continuous and intermittent bolus orogastric feedings are strategies used in infants unable to tolerate normal feeds. To determine the effects of feeding modality on protein synthesis in different tissues, neonatal pigs received a balanced formula by orogastric tube either as an intermittent bolus feed every 4 h or as a continuous infusion, or were fasted overnight. Compared to fasting, protein synthesis in gastrocnemius, masseter, and soleus muscles, left ventricle, liver, pancreas, jejunum, and kidney increased in bolus and continuously fed pigs, but the greatest increase occurred after a bolus meal. Tuberous sclerosis complex (TSC2), the proline-rich AKT substrate of 40 kDa (PRAS40), eukaryotic initiation factor (eIF) 4E binding protein (4EBP1) and rp S6 kinase 1 (S6K1) phosphorylation in all tissues and the proportion of ribosomal protein S4 in liver polysomes were enhanced 90 minutes following the bolus meal, but not immediately before the meal or during continuous feeding. Eukaryotic elongation factor 2 (eEF2) and eIF2α phosphorylation were unaffected by feeding. These results suggest that intermittent bolus feeding increases protein synthesis in muscles of different fiber types and visceral tissues to a greater extent than continuous feeding by stimulating translation initiation.
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影响因子：
4.2
作者：
Anthony, JC;Anthony, TG;Jefferson, LS
通讯作者：
Jefferson, LS
DOI：
10.1152/ajpendo.00351.2005
发表时间：
2006-02-01
影响因子：
5.1
作者：
Frank, JW;Escobar, J;Davis, TA
通讯作者：
Davis, TA
DOI：
10.1080/07315724.2000.10718080
发表时间：
2000-12-01
影响因子：
3.5
作者：
Dollberg, S;Kuint, J;Mimouni, FB
通讯作者：
Mimouni, FB
影响因子：
4.2
作者：
Gazzaneo, Maria C.;Suryawan, Agus;Davis, Teresa A.
通讯作者：
Davis, Teresa A.
影响因子：
3.6
作者：
Gazzaneo, Maria C.;Orellana, Renan A.;Davis, Teresa A.
通讯作者：
Davis, Teresa A.