Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs.
Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs.
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DOI：
10.1262/jrd.2016-113
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发表时间：
2016-12-20
期刊：
影响因子：
--
通讯作者：
Kikuchi K
中科院分区：
文献类型：
--
作者：
Nakai M;Ito J;Suzuki SI;Fuchimoto DI;Sembon S;Suzuki M;Noguchi J;Kaneko H;Onishi A;Kashiwazaki N;Kikuchi K
在猪中，卵胞浆内单精子注射（ICSI）后的胚胎生产效率仍然很低，因为正常受精经常失败，这涉及到两个极体和两个原核的形成。为了阐明原因，我们假设ICSI不能正确触发精子诱导的受精事件，特别是细胞内Ca2+信号传导，也称为Ca2+振荡。我们还怀疑使用体外成熟卵母细胞可能会对精子注射卵母细胞的受精事件和胚胎发育产生负面影响。因此，我们比较了钙离子振荡的模式，卵母细胞激活和正常受精的效率，以及胚胎发育到囊胚阶段之间的体内或体外成熟的卵母细胞ICSI或体外受精（IVF）。出乎意料的是，我们发现，钙振荡的模式，如钙离子上升的频率和幅度，在卵母细胞ICSI后是相似的卵母细胞体外受精后，无论卵母细胞的来源。然而，半数卵母细胞在ICSI后未能被激活，并且没有显示出Ca2+振荡。此外，正常受精卵母细胞的胚胎发育减少时，在体外成熟的卵母细胞被使用，无论采用的受精方法。这些结果表明，ICSI后的低胚胎生产效率主要是由于体外成熟卵母细胞的发育能力差，缺乏Ca2+振荡，而不是振荡的模式。
In pigs, the efficiency of embryo production after intracytoplasmic sperm injection (ICSI) is still low because of frequent failure of normal fertilization, which involves formation of two polar bodies and two pronuclei. To clarify the reasons for this, we hypothesized that ICSI does not properly trigger sperm-induced fertilization events, especially intracellular Ca2+ signaling, also known as Ca2+ oscillation. We also suspected that the use of in vitro-matured oocytes might negatively affect fertilization events and embryonic development of sperm-injected oocytes. Therefore, we compared the patterns of Ca2+ oscillation, the efficiency of oocyte activation and normal fertilization, and embryo development to the blastocyst stage among in vivo- or in vitro-matured oocytes after ICSI or in vitro fertilization (IVF). Unexpectedly, we found that the pattern of Ca2+ oscillation, such as the frequency and amplitude of Ca2+ rises, in oocytes after ICSI was similar to that in oocytes after IVF, irrespective of the oocyte source. However, half of the oocytes failed to become activated after ICSI and showed no Ca2+ oscillation. Moreover, the embryonic development of normal fertilized oocytes was reduced when in vitro-matured oocytes were used, irrespective of the fertilization method employed. These findings suggest that low embryo production efficiency after ICSI is attributable mainly to poor developmental ability of in vitro-matured oocytes and a lack of Ca2+ oscillation, rather than the pattern of oscillation.
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