The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata.
The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata.
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DOI：
10.1371/journal.pone.0011760
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发表时间：
2010-07-23
期刊：
影响因子：
3.7
通讯作者：
Su TT
中科院分区：
文献类型：
--
作者：
Su TT
原肠胚形成在囊胚中期转变的开始可以伴随胚胎细胞周期的深刻变化，包括间隙期的引入和从母体到合子控制的转变。对非洲爪蟾和果蝇胚胎的研究还发现，MBT（或果蝇中的等效物MZT）前后，细胞周期对DNA损伤的反应不同。DNA检查点在非洲爪蟾卵裂周期中不存在，但在MBT期间获得。果蝇卵裂核在DNA损伤的情况下进入一个失败的有丝分裂，而后MZT细胞延迟进入有丝分裂。尽管爪蟾和果蝇的属性使它们成为胚胎细胞周期研究的主力，但它们很难代表存在的各种动物形式。为了探讨DNA损伤反应在一个遥远的门的发育变化，我研究了烷化剂，甲基甲磺酸（MMS），对胚胎的棘海葵。Hydroactinia胚胎被发现不同于非洲爪蟾胚胎的能力，以应对DNA损伤剂在早期卵裂，但非洲爪蟾和果蝇胚胎在获得更强的DNA损伤反应和更大的抗性杀死MMS后的原肠胚形成的发病。这是首次对刺胞动物门DNA损伤反应的研究。
The onset of gastrulation at the Mid-Blastula Transition can accompany profound changes in embryonic cell cycles including the introduction of gap phases and the transition from maternal to zygotic control. Studies in Xenopus and Drosophila embryos have also found that cell cycles respond to DNA damage differently before and after MBT (or its equivalent, MZT, in Drosophila). DNA checkpoints are absent in Xenopus cleavage cycles but are acquired during MBT. Drosophila cleavage nuclei enter an abortive mitosis in the presence of DNA damage whereas post-MZT cells delay the entry into mitosis. Despite attributes that render them workhorses of embryonic cell cycle studies, Xenopus and Drosophila are hardly representative of diverse animal forms that exist. To investigate developmental changes in DNA damage responses in a distant phylum, I studied the effect of an alkylating agent, Methyl Methanesulfonate (MMS), on embryos of Hydractinia echinata. Hydractinia embryos are found to differ from Xenopus embryos in the ability to respond to a DNA damaging agent in early cleavage but are similar to Xenopus and Drosophila embryos in acquiring stronger DNA damage responses and greater resistance to killing by MMS after the onset of gastrulation. This represents the first study of DNA damage responses in the phylum Cnidaria.
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DOI：
10.1668/0003-1569(2001)041
发表时间：
2001-04-01
期刊：
AMERICAN ZOOLOGIST
影响因子：
--
作者：
Kramer, DL;McLaughlin, RL
通讯作者：
McLaughlin, RL
影响因子：
64.5
作者：
Takada, S;Kelkar, A;Theurkauf, WE
通讯作者：
Theurkauf, WE
影响因子：
21.3
作者：
Sibon, OCM;Kelkar, A;Theurkauf, WE
通讯作者：
Theurkauf, WE
影响因子：
3.7
作者：
Le Bouffant, Ronan;Boulben, Sandrine;Morales, Julia
通讯作者：
Morales, Julia
影响因子：
8
作者：
Le Bouffant, R.;Cormier, P.;Mulner-Lorillon, O.
通讯作者：
Mulner-Lorillon, O.