Evaluation of Grower-Friendly, Science-Based Sampling Approaches for the Detection of Salmonella in Ponds Used for Irrigation of Fresh Produce.
Evaluation of Grower-Friendly, Science-Based Sampling Approaches for the Detection of Salmonella in Ponds Used for Irrigation of Fresh Produce.
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评估用于新鲜农产品灌溉的池塘中沙门氏菌检测的种植者友好型、基于科学的采样方法。
DOI：
10.1089/fpd.2018.2441
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发表时间：
2018
影响因子：
2.8
通讯作者：
Levy,Karen
中科院分区：
文献类型：
--
作者：
Lee,Debbie;Tertuliano,Moukaram;Vellidis,George;Harris,Casey;Grossman,MarissaK;Rajeev,Sreekumari;Levy,Karen
人们认识到，灌溉水源有助于收获前污染的产品，导致了新的法规测试微生物水质。为了更好地识别污染问题，依赖灌溉池塘的种植者需要指导如何以及在哪里收集水样进行测试。在这项研究中，我们评估了几种采样策略，以确定沙门氏菌和大肠杆菌污染的五个池塘用于灌溉的农产品农场在南部格鲁吉亚。Salmonella和E.在19个月的研究期间，在所有池塘中定期检测到大肠杆菌，总的流行率和浓度在夏末和初秋增加。在507份水样中，沙门氏菌阳性217份（42.8%），几何平均浓度（GM）为0.06最大可能数（MPN）/100 mL，大肠杆菌阳性442份（87.1%）。大肠杆菌，GM为6.40 MPN/100 mL。我们发现在沙门氏菌或E中没有显著差异。当与我们认为是黄金标准的水泵取水口的结果进行比较时，在最接近水泵取水口的堤岸取样与在池塘周边堤岸取样之间的大肠杆菌检测率或浓度。然而，从最接近取水口的河岸采集的样本与取水口的一致性水平（Cohen's kappa统计值= 0.53; p <0.001）高于从池塘周边采集的样本（kappa = 0.34; p = 0.009）。大肠菌群与沙门氏菌检出率增加相关（比值比= 1.31; 95%可信区间= 1.10 - 1.56）。所有的池塘都符合E的生产安全规则标准。大肠杆菌，但也检出沙门氏菌。这项研究的结果为种植者和监管机构提供了有关灌溉池塘病原体检测的重要信息，并为地表水采样的最佳实践提供了信息。
The recognition that irrigation water sources contribute to preharvest contamination of produce has led to new regulations on testing microbial water quality. To best identify contamination problems, growers who depend on irrigation ponds need guidance on how and where to collect water samples for testing. In this study, we evaluated several sampling strategies to identifySalmonellaandEscherichia colicontamination in five ponds used for irrigation on produce farms in southern Georgia. BothSalmonellaandE. coliwere detected regularly in all the ponds over the 19-month study period, with overall prevalence and concentrations increasing in late summer and early fall. Of 507 water samples, 217 (42.8%) were positive forSalmonella, with a very low geometric mean (GM) concentration of 0.06 most probable number (MPN)/100 mL, and 442 (87.1%) tested positive forE. coli, with a GM of 6.40 MPN/100 mL. We found no significant differences inSalmonellaorE. colidetection rates or concentrations between sampling at the bank closest to the pump intake versus sampling from the bank around the pond perimeter, when comparing with results from the pump intake, which we considered our gold standard. However, samples collected from the bank closest to the intake had a greater level of agreement with the intake (Cohen's kappa statistic = 0.53;p< 0.001) than the samples collected around the pond perimeter (kappa = 0.34;p= 0.009).E. coliconcentrations were associated with increased odds ofSalmonelladetection (odds ratio = 1.31; 95% confidence interval = 1.10–1.56). All the ponds would have met the Produce Safety Rule standards forE. coli, althoughSalmonellawas also detected. Results from this study provide important information to growers and regulators about pathogen detection in irrigation ponds and inform best practices for surface water sampling.
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