Neuronal nitric oxide synthase immunopositive neurons in cat claustrum—a light and electron microscopic study
Neuronal nitric oxide synthase immunopositive neurons in cat claustrum—a light and electron microscopic study
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猫屏状体中神经元一氧化氮合酶免疫阳性神经元——光和电子显微镜研究
DOI：
10.1007/s10735-008-9184-z
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发表时间：
2008
影响因子：
3.2
通讯作者：
W. Ovtscharoff
中科院分区：
文献类型：
--
作者：
D. Hinova;L. Edelstein;A. Paloff;S. Hristov;V. Papantchev;W. Ovtscharoff
一氧化氮是一种独特的神经递质，参与机体的许多生理和病理过程。然而，关于猫背屏状体（DC）神经元一氧化氮合酶免疫反应（nNOS-ir）神经元和纤维的数据很少。在这方面，本研究的目的是：(1)证明nNOS-ir在DC的神经元和纤维中；(2)描述其光镜形态和分布；(3)观察和分析nNOS-ir神经元、纤维和突触末梢的超微结构；(4)验证nNOS-ir神经元是否属于特定的闭叶神经元亚群；(5)验证nNOS-ir神经元在整个DC中是否具有特定的组织模式。采用亲和素-生物素-过氧化物酶络合物法对nNOS-ir进行验证。DC各部位nNOS神经元和纤维均呈免疫阳性。光镜下nNOS-ir神经元在形状和大小上存在差异。根据孔径大小，将其分为小孔径（直径小于15 μm）、中孔径（直径16 ~ 20 μm）和大孔径（直径大于21 μm）三组。nNOS-ir神经元部分呈浅染，部分呈深染。电镜下观察到神经元、树突和终末钮扣中均有免疫产物。不同类型的nNOS-ir神经元的超微结构特征不同。发现三种类型的nNOS-ir突触钮扣。作为结论，我们希望本研究将有助于更好地了解猫的DC功能，并且所提供的一些数据可以外推到其他哺乳动物，包括人类。
Nitric oxide is a unique neurotransmitter, which participates in many physiological and pathological processes in the organism. Nevertheless there are little data about the neuronal Nitric Oxide Synthase immunoreactive (nNOS-ir) neurons and fibers in the dorsal claustrum (DC) of a cat. In this respect the aims of this study were: (1) to demonstrate nNOS-ir in the neurons and fibers of the DC; (2) to describe their light microscopic morphology and distribution; (3) to investigate and analyze the ultrastructure of the nNOS-ir neurons, fibers and synaptic terminals; (4) to verify whether the nNOS-ir neurons consist a specific subpopulation of claustral neurons; (5) to verify whether the nNOS-ir neurons have a specific pattern of organization throughout the DC. For demonstration of the nNOS-ir the Avidin-Biotin-Peroxidase Complex method was applied. Immunopositive for nNOS neurons and fibers were present in all parts of DC. On the light microscope level nNOS-ir neurons were different in shape and size. According to the latter they were divided into three groups—small (with diameter under 15 μm), medium-sized (with diameter from 16 to 20 μm) and large (with diameter over 21 μm). Some of nNOS-ir neurons were lightly-stained while others were darkly-stained. On the electron microscope level the immunoproduct was observed in neurons, dendrites and terminal boutons. Different types of nNOS-ir neurons differ according to their ultrastructural features. Three types of nNOS-ir synaptic boutons were found. As a conclusion we hope that the present study will contribute to a better understanding of the functioning of the DC in cat and that some of the data presented could be extrapolated to other mammals, including human.
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DOI：
10.1073/pnas.88.14.6368
发表时间：
1991-07-01
影响因子：
11.1
作者：
DAWSON, VL;DAWSON, TM;SNYDER, SH
通讯作者：
SNYDER, SH
影响因子：
1.6
作者：
L. Edelstein;F. Denaro
通讯作者：
L. Edelstein;F. Denaro
影响因子：
4.8
作者：
Wada, K;Chatzipanteli, K;Dietrich, WD
通讯作者：
Dietrich, WD
DOI：
10.1073/pnas.88.17.7797
发表时间：
1991-09-01
影响因子：
11.1
作者：
DAWSON, TM;BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH