Progesterone alleviates neural behavioral deficits and demyelination with reduced degeneration of oligodendroglial cells in cuprizone-induced mice.
Progesterone alleviates neural behavioral deficits and demyelination with reduced degeneration of oligodendroglial cells in cuprizone-induced mice.
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黄体酮可减轻铜宗诱导小鼠的神经行为缺陷和脱髓鞘，并减少少突胶质细胞的变性
DOI：
10.1371/journal.pone.0054590
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yao ZX
中科院分区：
文献类型：
--
作者：
Ye JN;Chen XS;Su L;Liu YL;Cai QY;Zhan XL;Xu Y;Zhao SF;Yao ZX
脱髓鞘广泛发生在神经退行性疾病中。Progestrin具有神经保护作用，已知可降低临床评分和炎症反应。Progestrin还促进实验性自身免疫性脑脊髓炎和铜腙诱导的脱髓鞘脑的髓鞘再生。然而，孕酮是否能减轻铜唑酮诱导的小鼠神经行为缺陷和少突胶质细胞变性的脱髓鞘仍不清楚。在这项研究中，用0.2%的cuprizone喂养小鼠以诱导脱髓鞘，并用孕酮处理以测试其对神经行为缺陷、脱髓鞘和少突胶质细胞变性的潜在保护作用。我们的研究结果表明，与溶剂处理的cuprizone组相比，孕酮处理后的神经行为缺陷明显减轻，这是通过平均体重的变化以及在旷场和旋转杆试验期间的活动来评估的。Luxol固蓝染色、MBP免疫组化染色和电镜观察显示，Progressive治疗减轻了脱髓鞘。孕酮组小鼠脑内TUNEL和Caspase-3阳性凋亡细胞明显减少，PDGFRα、Olig 2、Sox 10和CC-1抗体阳性的少突胶质细胞明显增多。这些结果表明，孕酮可以减轻铜腙诱导的小鼠神经行为缺陷和少突胶质细胞变性的脱髓鞘。
Demyelination occurs widely in neurodegenerative diseases. Progesterone has neuroprotective effects, is known to reduce the clinical scores and the inflammatory response. Progesterone also promotes remyelination in experimental autoimmune encephalomyelitis and cuprizone-induced demyelinating brain. However, it still remains unclear whether progesterone can alleviate neural behavioral deficits and demyelination with degeneration of oligodendroglial cells in cuprizone-induced mice. In this study, mice were fed with 0.2% cuprizone to induce demyelination, and treated with progesterone to test its potential protective effect on neural behavioral deficits, demyelination and degeneration of oligodendroglial cells. Our results showed noticeable alleviation of neural behavioral deficits following progesterone treatment as assessed by changes in average body weight, and activity during the open field and Rota-rod tests when compared with the vehicle treated cuprizone group. Progesterone treatment alleviated demyelination as shown by Luxol fast blue staining, MBP immunohistochemical staining, and electron microscopy. There was an obvious decrease in TUNEL and Caspase-3-positive apoptotic cells, and an increase in the number of oligodendroglial cells staining positive for PDGFRα, Olig2, Sox10 and CC-1 antibody in the brains of cuprizone-induced mice after progesterone administration. These results indicate that progesterone can alleviate neural behavioral deficits and demyelination against oligodendroglial cell degeneration in cuprizone-induced mice.
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影响因子：
10.5
作者：
Stolt, CC;Rehberg, S;Wegner, M
通讯作者：
Wegner, M
影响因子：
6.7
作者：
Kipp, M.;Norkus, A.;Krauspe, B.;Clarner, T.;Berger, K.;van der Valk, P.;Amor, S.;Beyer, C.
通讯作者：
Beyer, C.
影响因子：
56.9
作者：
KOENIG, HL;SCHUMACHER, M;BAULIEU, EE
通讯作者：
BAULIEU, EE
影响因子：
5
作者：
Copray, JCVM;Küst, BM;Boddeke, HWGM
通讯作者：
Boddeke, HWGM
影响因子：
4
作者：
JungTestas, I;Schumacher, M;Baulieu, EE
通讯作者：
Baulieu, EE