Lipid-Bicelle-Coated Microfluidics for Intracellular Delivery with Reduced Fouling.
Lipid-Bicelle-Coated Microfluidics for Intracellular Delivery with Reduced Fouling.
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DOI：
10.1021/acsami.0c11485
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发表时间：
2020-10-14
影响因子：
9.5
通讯作者：
Weiss PS
中科院分区：
文献类型：
--
作者：
Belling JN;Heidenreich LK;Park JH;Kawakami LM;Takahashi J;Frost IM;Gong Y;Young TD;Jackman JA;Jonas SJ;Cho NJ;Weiss PS
细胞内递送的创新技术正在开创基因编辑的新时代，使患者自己的细胞能够用于干细胞和免疫治疗。特别地，细胞挤压平台提供非常规形式的细胞内递送，使细胞通过微流体收缩变形以产生瞬时孔并使生物分子货物能够有效扩散。虽然这些设备是有前途的基因编辑平台，但由于细胞碎片的积累，它们需要频繁维护，限制了它们达到可扩展细胞疗法所需的吞吐量的潜力。随着这些细胞挤压技术的改进，需要开发具有更高通量和更长寿命的下一代平台，重要的是，避免细胞碎片的积累和因此的通道堵塞。在这里，我们报告了一种通用的策略，以基于非共价脂质双分子技术的脂质双层涂覆微流体装置的通道，这导致了在减少细胞粘附和蛋白质吸附方面的实质性改善。评价了脂质双层涂层的生物相容性，包括膜均匀性、对非特异性蛋白质吸附的钝化作用以及对多种细胞类型的细胞附着的抑制作用。这种表面功能化方法被应用于涂覆狭窄的微流体通道，用于荧光标记的葡聚糖和质粒DNA的细胞内递送，表明细胞碎片的积累显著减少。两者合计，我们的工作表明，脂质双胞是一个有用的工具，在细胞挤压装置中制造可降解的脂质双层涂层，从而减少非特异性污染和细胞堵塞，以提高性能。
Innovative technologies for intracellular delivery are ushering in a new era for gene editing, enabling the utilization of a patient’s own cells for stem cell and immunotherapies. In particular, cell-squeezing platforms provide unconventional forms of intracellular delivery, deforming cells through microfluidic constrictions to generate transient pores and to enable effective diffusion of biomolecular cargo. While these devices are promising gene-editing platforms, they require frequent maintenance due to the accumulation of cellular debris, limiting their potential for reaching the throughputs necessary for scalable cellular therapies. As these cell-squeezing technologies are improved, there is a need to develop next-generation platforms with higher throughput and longer lifespan, importantly, avoiding the buildup of cell debris and thus channel clogging. Here, we report a versatile strategy to coat the channels of microfluidic devices with lipid bilayers based on noncovalent lipid bicelle technology, which led to substantial improvements in reducing cell adhesion and protein adsorption. The antifouling properties of the lipid bilayer coating were evaluated, including membrane uniformity, passivation against nonspecific protein adsorption, and inhibition of cell attachment against multiple cell types. This surface functionalization approach was applied to coat constricted microfluidic channels for the intracellular delivery of fluorescently labeled dextran and plasmid DNA, demonstrating significant reductions in the accumulation of cell debris. Taken together, our work demonstrates that lipid bicelles are a useful tool to fabricate antifouling lipid bilayer coatings in cell-squeezing devices, resulting in reduced nonspecific fouling and cell clogging to improve performance.
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DOI：
10.1073/pnas.1917125117
发表时间：
2020-05-19
影响因子：
11.1
作者：
Belling, Jason N.;Heidenreich, Liv K.;Jonas, Steven J.
通讯作者：
Jonas, Steven J.
影响因子：
3.2
作者：
Carugo, Dario;Ankrett, Dyan N.;Hill, Martyn
通讯作者：
Hill, Martyn
DOI：
10.1002/jbm.10005
发表时间：
2002-04-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Liu, VA;Jastromb, WE;Bhatia, SN
通讯作者：
Bhatia, SN
影响因子：
6.1
作者：
Green, James V.;Kniazeva, Tatiana;Murthy, Shashi K.
通讯作者：
Murthy, Shashi K.
DOI：
10.1016/j.mattod.2018.03.002
发表时间：
2018-09
期刊：
Materials today (Kidlington, England)
影响因子：
--
作者：
Liu A;Islam M;Stone N;Varadarajan V;Jeong J;Bowie S;Qiu P;Waller EK;Alexeev A;Sulchek T
通讯作者：
Sulchek T