Genome-wide identification of ABA receptor PYL family and expression analysis of PYLs in response to ABA and osmotic stress in Gossypium.
Genome-wide identification of ABA receptor PYL family and expression analysis of PYLs in response to ABA and osmotic stress in Gossypium.
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作者：
Zhang G;Lu T;Miao W;Sun L;Tian M;Wang J;Hao F
脱落酸（ABA）受体吡拉菌素抗性1/PYR1样/ABA受体的调节成分（PYR1/PYL/RCAR）（为简单起见命名为PYL）是ABA信号传导的核心调节因子，并已在拟南芥和水稻中得到充分研究。然而，有关棉属 PYL 家族基因组组织、基因结构、系统发育、基因表达以及蛋白质与下游靶标相互作用的知识有限。对棉属 PYL 家族进行了全面分析，在二倍体祖先植物棉 (G. arboretum)、雷蒙德棉 (G. raimondii) 以及四倍体陆地棉 (G. hirsutum) 和海岛棉 (G. barbadense) 的基因组中分别鉴定出 21、20、40 和 39 个 PYL 基因。这些家族成员的物理特性、染色体位置、结构和系统发育的表征表明，Gossypium PYLs 在调查的棉花品种中相当保守。片段重复可能是促进PYLs扩展的主要力量，并且大多数PYLs在棉花的纯化选择下经历了进化。此外，GhPYL 基因的表达谱在组织中具有特异性。许多 GhPYL 基因的转录受到 ABA 处理的抑制和渗透压的诱导。在棉花中通过酵母-两种杂交方法，在存在和/或不存在 ABA 的情况下，许多 GhPYL 可以与 GhABI1A 或 GhABID 相互作用。
Abscisic acid (ABA) receptor pyrabactin resistance1/PYR1-like/regulatory components of ABA receptor (PYR1/PYL/RCAR) (named PYLs for simplicity) are core regulators of ABA signaling, and have been well studied in Arabidopsis and rice. However, knowledge is limited about the PYL family regarding genome organization, gene structure, phylogenesis, gene expression and protein interaction with downstream targets in Gossypium. A comprehensive analysis of the Gossypium PYL family was carried out, and 21, 20, 40 and 39 PYL genes were identified in the genomes from the diploid progenitor G. arboretum, G. raimondii and the tetraploid G. hirsutum and G. barbadense, respectively. Characterization of the physical properties, chromosomal locations, structures and phylogeny of these family members revealed that Gossypium PYLs were quite conservative among the surveyed cotton species. Segmental duplication might be the main force promoting the expansion of PYLs, and the majority of the PYLs underwent evolution under purifying selection in Gossypium. Additionally, the expression profiles of GhPYL genes were specific in tissues. Transcriptions of many GhPYL genes were inhibited by ABA treatments and induced by osmotic stress. A number of GhPYLs can interact with GhABI1A or GhABID in the presence and/or absence of ABA by the yeast-two hybrid method in cotton.
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影响因子：
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作者：
González-Guzmán M;Rodríguez L;Lorenzo-Orts L;Pons C;Sarrión-Perdigones A;Fernández MA;Peirats-Llobet M;Forment J;Moreno-Alvero M;Cutler SR;Albert A;Granell A;Rodríguez PL
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