Activation of ABA Receptors Gene GhPYL9-11A Is Positively Correlated with Cotton Drought Tolerance in Transgenic Arabidopsis.
Activation of ABA Receptors Gene GhPYL9-11A Is Positively Correlated with Cotton Drought Tolerance in Transgenic Arabidopsis.
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转基因拟南芥ABA受体基因GhPYL9-11A的激活与棉花耐旱性正相关
DOI：
10.3389/fpls.2017.01453
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Liang C;Liu Y;Li Y;Meng Z;Yan R;Zhu T;Wang Y;Kang S;Ali Abid M;Malik W;Sun G;Guo S;Zhang R
脱落酸（ABA）受体pyrabactin resistance-like protein （PYLs）对ABA的敏感性被认为是激活ABA信号通路以应对非生物胁迫的最重要因素。然而，目前尚不清楚PYL是介导棉花对干旱胁迫反应的关键ABA受体。本文报道了棉花干旱胁迫下ABA受体GhPYL9-11A的鉴定和鉴定。结果表明，外源ABA对GhPYL9-11A具有很强的诱导作用。GhPYL9-11A以不依赖aba的方式结合蛋白磷酸酶2c （pp2c）。此外，GhPYL-11A-PP2C相互作用被门锁区脯氨酸（P84）和组氨酸（H111）突变部分破坏。过表达GhPYL9-11A的转基因拟南芥植株在种子萌发和幼苗早期对ABA敏感。此外，与野生型相比，转基因拟南芥根系生长加快，干旱胁迫相关基因上调，证实了GhPYL9-11A在非生物胁迫耐受中的潜在作用。干旱处理下，GhPYL9-11A在抗旱棉花品种中的平均表达量高于抗旱棉花品种。综上所述，调控GhPYL9-11A的表达可能是培育耐旱棉花品种的有效策略。
The sensitivity to abscisic acid (ABA) by its receptors, pyrabactin resistance-like proteins (PYLs), is considered a most important factor in activating the ABA signal pathway in response to abiotic stress. However, it is still unknown which PYL is the crucial ABA receptor mediating response to drought stress in cotton (Gossypium hirsutum L.). Here, we reported the identification and characterization of highly induced ABA receptor GhPYL9-11A in response to drought in cotton. It is observed that GhPYL9-11A was highly induced by ABA treatment. GhPYL9-11A binds to protein phosphatase 2Cs (PP2Cs) in an ABA-independent manner. Moreover, the GhPYL-11A-PP2C interactions are partially disrupted by mutations, proline (P84) and histidine (H111), in the gate-latch region. Transgenic Arabidopsis overexpressing GhPYL9-11A plants were hypersensitive to ABA during seed germination and early seedling stage. Further, the increased in root growth and up regulation of drought stress-related genes in transgenic Arabidopsis as compared to wild type confirmed the potential role of GhPYL9-11A in abiotic stress tolerance. Consistently, the expression level of GhPYL9-11A is on average higher in drought-tolerant cotton cultivars than in drought-sensitive cottons under drought treatment. In conclusion, the manipulation of GhPYL9-11A expression could be a useful strategy for developing drought-tolerant cotton cultivars.
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影响因子：
4.4
作者：
Lin L;Pierce GJ;Bowers JE;Estill JC;Compton RO;Rainville LK;Kim C;Lemke C;Rong J;Tang H;Wang X;Braidotti M;Chen AH;Chicola K;Collura K;Epps E;Golser W;Grover C;Ingles J;Karunakaran S;Kudrna D;Olive J;Tabassum N;Um E;Wissotski M;Yu Y;Zuccolo A;ur Rahman M;Peterson DG;Wing RA;Wendel JF;Paterson AH
通讯作者：
Paterson AH
脱甲酸受体的激素信号传导的栅极锁锁机制。
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
46.9
作者：
Li, Fuguang;Fan, Guangyi;Yu, Shuxun
通讯作者：
Yu, Shuxun
DOI：
10.1155/2016/6230825
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Liu Y;Su D;Zhang L;Wei S;Liu K;Peng M;Li H;Song Y
通讯作者：
Song Y
影响因子：
64.8
作者：
Miyazono, Ken-ichi;Miyakawa, Takuya;Tanokura, Masaru
通讯作者：
Tanokura, Masaru