Tomato PYR/PYL/RCAR abscisic acid receptors show high expression in root, differential sensitivity to the abscisic acid agonist quinabactin, and the capability to enhance plant drought resistance.
Tomato PYR/PYL/RCAR abscisic acid receptors show high expression in root, differential sensitivity to the abscisic acid agonist quinabactin, and the capability to enhance plant drought resistance.
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DOI：
10.1093/jxb/eru219
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发表时间：
2014-08
影响因子：
6.9
通讯作者：
Rodríguez PL
中科院分区：
文献类型：
--
作者：
González-Guzmán M;Rodríguez L;Lorenzo-Orts L;Pons C;Sarrión-Perdigones A;Fernández MA;Peirats-Llobet M;Forment J;Moreno-Alvero M;Cutler SR;Albert A;Granell A;Rodríguez PL
化学和转基因方法可以通过作物PYR/PYL ABA受体激活ABA信号；quinabactin可选择性激活番茄ABA受体，单体型受体过表达可增强植株抗旱性。脱落酸（ABA）在植物对生物和非生物胁迫的反应中起着至关重要的作用。可持续粮食生产面临若干关键挑战，特别是培育水资源利用效率更高、耐旱能力更强的新品种。不同的研究表明拟南芥PYR/PYL/RCAR ABA受体在提高植物抗旱性方面具有潜在的应用前景。因此，作物中同源基因的功能表征为农业带来了希望。在此鉴定了整套番茄（Solanum lycopersicum）的PYR/PYL/RCAR ABA受体。从15个推测的番茄ABA受体中，14个可归为3个亚家族，与相应的拟南芥亚家族相关良好。PYR/PYL/RCAR基因在番茄根部高表达，部分基因在叶片和果实组织中显著表达。通过与拟南芥和番茄枝A蛋白磷酸酶2c型（pp2c）的相互作用以及磷酸酶抑制研究，对番茄受体的功能进行了表征。番茄受体能够以ABA依赖的方式抑制A枝pp2c的活性，并且至少有三种受体对ABA激动剂quinabactin敏感，从而抑制番茄种子的萌发。事实上，quinabactin诱导的ABA信号的化学激活能够激活应激反应基因。二聚体和单体番茄受体在拟南芥植物细胞中都有功能，但只有单体型受体的过表达才能增强抗旱性。总之，基因表达分析以及化学和转基因方法揭示了番茄PYR/PYL/RCAR ABA受体的独特特性，这些特性可能具有生物技术意义。
Chemical and transgenic approaches can activate ABA signalling via crop PYR/PYL ABA receptors; quinabactin can selectively activate tomato ABA receptors, and overexpression of monomeric-type receptors confers enhanced plant drought resistance. Abscisic acid (ABA) plays a crucial role in the plant’s response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efficiency and drought tolerance. Different studies have shown the potential applications of Arabidopsis PYR/PYL/RCAR ABA receptors to enhance plant drought resistance. Consequently the functional characterization of orthologous genes in crops holds promise for agriculture. The full set of tomato (Solanum lycopersicum) PYR/PYL/RCAR ABA receptors have been identified here. From the 15 putative tomato ABA receptors, 14 of them could be grouped in three subfamilies that correlated well with corresponding Arabidopsis subfamilies. High levels of expression of PYR/PYL/RCAR genes was found in tomato root, and some genes showed predominant expression in leaf and fruit tissues. Functional characterization of tomato receptors was performed through interaction assays with Arabidopsis and tomato clade A protein phosphatase type 2Cs (PP2Cs) as well as phosphatase inhibition studies. Tomato receptors were able to inhibit the activity of clade A PP2Cs differentially in an ABA-dependent manner, and at least three receptors were sensitive to the ABA agonist quinabactin, which inhibited tomato seed germination. Indeed, the chemical activation of ABA signalling induced by quinabactin was able to activate stress-responsive genes. Both dimeric and monomeric tomato receptors were functional in Arabidopsis plant cells, but only overexpression of monomeric-type receptors conferred enhanced drought resistance. In summary, gene expression analyses, and chemical and transgenic approaches revealed distinct properties of tomato PYR/PYL/RCAR ABA receptors that might have biotechnological implications.
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影响因子：
64.8
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影响因子：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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影响因子：
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