In Vivo Detection and Measurement of Aortic Aneurysm and Dissection in Mouse Models Using Microcomputed Tomography with Contrast Agent
In Vivo Detection and Measurement of Aortic Aneurysm and Dissection in Mouse Models Using Microcomputed Tomography with Contrast Agent
复制标题
使用造影剂显微计算机断层扫描在小鼠模型中体内检测和测量主动脉瘤和夹层
DOI：
10.1155/2019/5940301
复制
发表时间：
2019-03
影响因子：
--
通讯作者：
Jing Zaiping
中科院分区：
文献类型：
--
作者：
Zhu Guanglang;Sun Huiying;Wang Jiannan;Zhao Zhiqing;Bao Junmin;Feng Rui;Zhou Jian;Jing Zaiping
目的探讨血管内造影剂Micro-CT在主动脉瘤和夹层小鼠模型主动脉成像及动态监测中的应用价值。材料与方法实验采用健康小鼠和主动脉夹层小鼠。正在发展主动脉夹层的小鼠和健康小鼠在注射ESTRON nano 12000后进行显微CT成像。测量健康小鼠主动脉中的时间依赖性信号增强（分别在静脉注射造影剂后1、2、3、6和12小时）以确认主动脉的最佳成像时间。研究了各种造影剂剂量（分别为70、100和150 μl/25 g小鼠），以确定主动脉成像所需的最佳剂量。小鼠在动脉瘤和夹层发病后1、14和28天进行微型CT扫描，监测主动脉的动态变化过程。用苏木精-伊红染色法对小鼠腹主动脉进行染色，测量主动脉直径，并与显微CT测量结果进行比较。结果主动脉内时间依赖性信号增强显示造影剂具有较长的血液半衰期，约为6 h，在注射后2 h达到峰值增强。每只25 g小鼠注射100 μl Atrium Tron nano 12000可有效观察主动脉。Micro-CT结合对比剂可以动态监测主动脉瘤和夹层小鼠模型主动脉的变化过程。将从体内显微CT成像获得的动脉瘤直径值与从组织学获得的动脉瘤直径值进行比较，并显示出显著相关性（R2 = 0.96）。结论这些数据表明，在体内micro-CT是一种准确和可行的技术来检测主动脉瘤或夹层在小鼠模型中，和micro-CT技术，使用创新的造影剂NATIOTRON纳米12000允许动态监测各种过程，如主动脉重构的纵向研究。
Objectives The aim of this study was to evaluate the potential of microcomputed tomography (micro-CT) using the intravascular contrast agent ExiTron nano 12000 for aorta imaging and monitoring the dynamic changing process of the aorta in mouse models with aortic aneurysm and dissection. Materials and Methods Experiments were performed on healthy mice and mice with aortic dissection. Mice that were developing aortic dissection and healthy mice underwent micro-CT imaging after injection of ExiTron nano 12000. Time-dependent signal enhancement (at 1, 2, 3, 6, and 12 hours after intravenous injection of the contrast agent, respectively) in the aorta of healthy mice was measured to confirm the optimal imaging time of aorta. Various contrast agent doses (70, 100, and 150 μl per 25 g mouse, respectively) were investigated to determine the optimal required dose for imaging of the aorta. The mice were scanned with micro-CT at 1, 14, and 28 days after onset of aneurysm and dissection to monitor the dynamic changing process of the aorta. Mouse aortas were stained with hematoxylin and eosin staining, and the diameter of the aorta was measured and compared with those obtained by micro-CT. Results Time-dependent signal enhancement in the aorta shows that the contrast agent has a long blood half-life of 6 hours, with a peak enhancement at 2 hours after injection. Injection of 100 μl ExiTron nano 12000 per 25 g mouse allows for effective visualization of the aorta. Micro-CT combined with contrast agent can monitor the changing process of the aorta in the mouse model of aortic aneurysm and dissection dynamically. The values of the diameter of the aortas obtained from the in vivo micro-CT imaging were compared with those obtained from histology and showed a significant correlation (R2 = 0.96). Conclusions These data demonstrate that in vivo micro-CT is an accurate and feasible technique to detect aortic aneurysm or dissection in a mouse model, and the micro-CT technique using the innovative contrast agent ExiTron nano 12000 allows for monitoring various processes dynamically such as aortic remodeling in longitudinal studies.
登录
查看更多内容
影响因子：
--
作者：
Ashton, Jeffrey R.;Befera, Nicholas;Clark, Darin;Qi, Yi;Mao, Lan;Rockman, Howard A.;Johnson, G. Allan;Badea, Cristian T.
通讯作者：
Badea, Cristian T.
影响因子：
9.6
作者：
Mészáros, I;Mórocz, J;Szép, L
通讯作者：
Szép, L
影响因子：
6.7
作者：
M. Wurnig;Y. Tsushima;M. Weiger;W. Jungraithmayr;A. Boss
通讯作者：
M. Wurnig;Y. Tsushima;M. Weiger;W. Jungraithmayr;A. Boss
DOI：
10.1007/s00424-014-1625-9
发表时间：
2014-10
期刊：
Pflügers Archiv - European Journal of Physiology
影响因子：
--
作者：
Hajar Fahkri;Bingbing Zhang;Abul Fajol;N. Hernando;B. Elvira;J. Mannheim;B. Pichler;C. Daniel;K. Amann;A. Hirao;J. Haight;T. Mak;F. Lang;M. Föller
通讯作者：
Hajar Fahkri;Bingbing Zhang;Abul Fajol;N. Hernando;B. Elvira;J. Mannheim;B. Pichler;C. Daniel;K. Amann;A. Hirao;J. Haight;T. Mak;F. Lang;M. Föller
影响因子：
3.1
作者：
Suckow, Chris E.;Stout, David B.
通讯作者：
Stout, David B.