Anatomical and functional imaging of myocardial infarction in mice using micro-CT and eXIA 160 contrast agent.
Anatomical and functional imaging of myocardial infarction in mice using micro-CT and eXIA 160 contrast agent.
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DOI：
10.1002/cmmi.1557
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发表时间：
2014-03
影响因子：
--
通讯作者：
Badea, Cristian T.
中科院分区：
文献类型：
--
作者：
Ashton, Jeffrey R.;Befera, Nicholas;Clark, Darin;Qi, Yi;Mao, Lan;Rockman, Howard A.;Johnson, G. Allan;Badea, Cristian T.
非侵入性小动物成像技术对于评估心脏病和潜在的治疗方法至关重要。最近开发了一种新型临床前碘化造影剂eXIA 160，并对其进行了微ct心脏成像评估。eXIA 160在注射后立即在血液和组织之间形成强烈的对比，随后随着时间的推移被心肌和其他代谢活跃组织吸收。我们重点研究了eXIA的这些特性，并展示了它在小鼠心肌梗死成像中的应用。5只C57BL/6小鼠在LAD冠状动脉结扎后约2周成像。6只C57BL/6小鼠作为对照。注射eXIA 160后，血液和心肌之间的增强差异约340 HU，通过4D微ct扫描回顾性门控估计心功能。注射后4 h，正常灌注心肌与血液对比差约140 HU，梗死心肌无增强。这些差异允许通过双能微ct定量梗死面积。体内微spect成像和离体TTC染色验证了微ct的发现。micro-CT和SPECT测量梗死的均方根误差为2.7%，micro-CT和TTC测量梗死的均方根误差为4.7%。因此，使用eXIA 160的micro-CT可以为评估心肌梗死和潜在治疗方法的临床前研究提供形态学和功能数据。eXIA 160作为小鼠其他代谢活跃组织的CT分子成像工具的潜在用途有待进一步研究。
Non-invasive small animal imaging techniques are essential for evaluation of cardiac disease and potential therapeutics. A novel preclinical iodinated contrast agent called eXIA 160 has recently been developed, which has been evaluated for micro-CT cardiac imaging. eXIA 160 creates strong contrast between blood and tissue immediately after its injection and is subsequently taken up by the myocardium and other metabolically active tissues over time. We focus on these properties of eXIA and show its use in imaging myocardial infarction in mice. Five C57BL/6 mice were imaged ~ 2 weeks after LAD coronary artery ligation. Six C57BL/6 mice were used as controls. Immediately after injection of eXIA 160, an enhancement difference between blood and myocardium of ~340 HU enabled cardiac function estimation via 4D micro-CT scanning with retrospective gating. Four hours post-injection, the healthy perfused myocardium had a contrast difference of ~140 HU relative to blood while the infarcted myocardium showed no enhancement. These differences allowed quantification of infarct size via dual energy micro-CT. In vivo micro-SPECT imaging and ex vivo TTC staining provided validation for the micro-CT findings. Root mean squared error of infarct measurements was 2.7% between micro-CT and SPECT, and 4.7% between micro-CT and TTC. Thus, micro-CT with eXIA 160 can be used to provide both morphological and functional data for preclinical studies evaluating myocardial infarction and potential therapies. Further studies are warranted to study the potential use of eXIA 160 as a CT molecular imaging tool for other metabolically active tissues in the mouse.
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影响因子：
6.7
作者：
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