Exploring the Genomic Traits of Non-toxigenic Vibrio parahaemolyticus Strains Isolated in Southern Chile.
Exploring the Genomic Traits of Non-toxigenic Vibrio parahaemolyticus Strains Isolated in Southern Chile.
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DOI：
10.3389/fmicb.2018.00161
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发表时间：
2018
影响因子：
5.2
通讯作者：
García K
中科院分区：
文献类型：
--
作者：
Castillo D;Pérez-Reytor D;Plaza N;Ramírez-Araya S;Blondel CJ;Corsini G;Bastías R;Loyola DE;Jaña V;Pavez L;García K
副溶血性弧菌是全球海产品传播性胃肠炎的主要原因。正如其他国家报告的那样，在智利大流行毒株的上升和下降之后，其他大流行后毒株与临床病例有关，包括缺乏主要毒素TDH和TRH的毒株。由于tdh和trh基因的存在与否已被用于诊断目的，并作为副溶血性弧菌分离株毒力的代表，因此了解缺乏毒素的副溶血性弧菌菌株的毒力对于检测水和海产品中存在的这些菌株以避免可能的食源性感染至关重要。在这项研究中，我们的基因组特征的四个环境和两个临床的非致突变株（tdh-，trh-和T3 SS 2-）。使用全基因组测序，系统发育和比较基因组分析，我们确定了智利南部副溶血性弧菌菌株的核心和泛基因组。基于核心基因组的系统发育树显示遗传多样性较低，但泛基因组分析显示，所有菌株都具有携带不同毒力和适应性因子或编码毒素（如Zot和RTX）的原噬菌体样元件的基因组岛。有趣的是，携带Zot样毒素的三种菌株具有不同的序列，尽管比对显示出与霍乱弧菌中发现的zot序列的一些保守区域。此外，我们确定了一个意想不到的多样性的遗传结构的T3 SS 1基因簇和存在的T3 SS 2基因簇在非大流行的环境菌株。我们的研究揭示了来自南太平洋的副溶血性弧菌菌株的多样性，这增加了我们目前对该生物全球多样性的了解。
Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. As reported in other countries, after the rise and fall of the pandemic strain in Chile, other post-pandemic strains have been associated with clinical cases, including strains lacking the major toxins TDH and TRH. Since the presence or absence of tdh and trh genes has been used for diagnostic purposes and as a proxy of the virulence of V. parahaemolyticus isolates, the understanding of virulence in V. parahaemolyticus strains lacking toxins is essential to detect these strains present in water and marine products to avoid possible food-borne infection. In this study, we characterized the genome of four environmental and two clinical non-toxigenic strains (tdh-, trh-, and T3SS2-). Using whole-genome sequencing, phylogenetic, and comparative genome analysis, we identified the core and pan-genome of V. parahaemolyticus of strains of southern Chile. The phylogenetic tree based on the core genome showed low genetic diversity but the analysis of the pan-genome revealed that all strains harbored genomic islands carrying diverse virulence and fitness factors or prophage-like elements that encode toxins like Zot and RTX. Interestingly, the three strains carrying Zot-like toxin have a different sequence, although the alignment showed some conserved areas with the zot sequence found in V. cholerae. In addition, we identified an unexpected diversity in the genetic architecture of the T3SS1 gene cluster and the presence of the T3SS2 gene cluster in a non-pandemic environmental strain. Our study sheds light on the diversity of V. parahaemolyticus strains from the southern Pacific which increases our current knowledge regarding the global diversity of this organism.
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影响因子：
14.9
作者：
Dhillon BK;Chiu TA;Laird MR;Langille MG;Brinkman FS
通讯作者：
Brinkman FS
影响因子：
2.2
作者：
Bej, AK;Patterson, DP;Kaysner, CA
通讯作者：
Kaysner, CA
弧菌性溶血性细胞生物学和致病性决定因素。
影响因子：
5.8
作者：
Broberg CA;Calder TJ;Orth K
通讯作者：
Orth K
影响因子：
3.1
作者：
Coelho, A;Andrade, JRC;DiRita, VJ
通讯作者：
DiRita, VJ
影响因子：
3
作者：
Blom J;Albaum SP;Doppmeier D;Pühler A;Vorhölter FJ;Zakrzewski M;Goesmann A
通讯作者：
Goesmann A