Expression of LIM-homeodomain transcription factors in the developing and mature mouse retina.
Expression of LIM-homeodomain transcription factors in the developing and mature mouse retina.
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DOI：
10.1016/j.gep.2013.12.001
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发表时间：
2014-01
影响因子：
1.2
通讯作者：
Gan, Lin
中科院分区：
文献类型：
--
作者：
Balasubramanian, Revathi;Bui, Andrew;Ding, Qian;Gan, Lin
LIM-homeodomain（LIM-HD）转录因子因其在中枢神经系统发育中的作用而受到广泛研究。它们的功能是几个发育事件的关键，如细胞增殖，分化和亚型特化。然而，它们在视网膜神经发生中的作用在很大程度上仍然未知。在这里，我们报告了一个详细的表达研究LIM-HD转录因子LHX 9和LHX 2，LHX 3和LHX 4，LHX 6在发育和成熟的小鼠视网膜使用免疫组织化学和原位杂交技术。我们发现LHX 9在视网膜神经节细胞层和内核层的早期发育阶段表达。我们还表明，LHX 9在成人视网膜的无长突细胞的子集中表达。已知LHX 2在发育期间在视网膜祖细胞中表达，并且在成年视网膜中在Müller神经胶质细胞和无长突细胞的子集中表达。我们发现LHX 2无长突细胞亚群不是胆碱能的，并且极少数LHX 2无长突细胞表达钙视黄蛋白。LHX 3和LHX 4在成人视网膜的双极细胞亚群中表达。LHX 6在胚胎期13.5（E13.5）开始在神经节细胞层和成神经细胞层中的细胞中表达，并在出生后继续在神经节细胞层和内核层中的细胞中表达，表明其可能在无长突细胞或其子集中表达。综上所述，我们对LIM-HD转录因子在小鼠视网膜发育过程中表达模式的全面分析将有助于进一步研究阐明其在视网膜细胞亚型分化中的生物学功能。
LIM-homeodomain (LIM-HD) transcription factors have been extensively studied for their role in the development of the central nervous system. Their function is key to several developmental events like cell proliferation, differentiation and subtype specification. However, their roles in retinal neurogenesis remain largely unknown. Here we report a detailed expression study of LIM-HD transcription factors LHX9 and LHX2, LHX3 and LHX4, and LHX6 in the developing and mature mouse retina using immunohistochemistry and in situ hybridization techniques. We show that LHX9 is expressed during the early stages of development in the retinal ganglion cell layer and the inner nuclear layer. We also show that LHX9 is expressed in a subset of amacrine cells in the adult retina. LHX2 is known to be expressed in retinal progenitor cells during development and in Müller glial cells and a subset of amacrine cells in the adult retina. We found that the LHX2 subset of amacrine cells is not cholinergic and that a very few of LHX2 amacrine cells express calretinin. LHX3 and LHX4 are expressed in a subset of bipolar cells in the adult retina. LHX6 is expressed in cells in the ganglion cell layer and the neuroblast layer starting at embryonic stage 13.5 (E13.5) and continues to be expressed in cells in the ganglion cell layer and inner nuclear layer, postnatally, suggesting its likely expression in amacrine cells or a subset thereof. Taken together, our comprehensive assay of expression patterns of LIM-HD transcription factors during mouse retinal development will help further studies elucidating their biological functions in the differentiation of retinal cell subtypes.
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影响因子：
3.7
作者：
Hägglund AC;Dahl L;Carlsson L
通讯作者：
Carlsson L
影响因子：
3.7
作者：
Duquette PM;Zhou X;Yap NL;MacLaren EJ;Lu JJ;Wallace VA;Chen HH
通讯作者：
Chen HH
DOI：
10.1073/pnas.1107488109
发表时间：
2012-03-20
影响因子：
11.1
作者：
de Melo, Jimmy;Miki, Katsuaki;Blackshaw, Seth
通讯作者：
Blackshaw, Seth
影响因子：
10.5
作者：
Dalal, Jasbir;Roh, Jee Hoon;Dougherty, Joseph D.
通讯作者：
Dougherty, Joseph D.
影响因子：
4.4
作者：
Lin, Ya-Ping;Ouchi, Yasuo;Watanabe, Sumiko
通讯作者：
Watanabe, Sumiko