Lentinan confers protection against type 1 diabetes by inducing regulatory T cell in spontaneous non-obese diabetic mice.
Lentinan confers protection against type 1 diabetes by inducing regulatory T cell in spontaneous non-obese diabetic mice.
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DOI：
10.1038/s41387-023-00233-7
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发表时间：
2023-04-08
影响因子：
6.1
通讯作者：
Han, Xiao
中科院分区：
文献类型：
--
作者：
Wu, Tijun;Cai, Zhi;Niu, Fandi;Qian, Bin;Sun, Peng;Yang, Nan;Pang, Jing;Mei, Hongliang;Chang, Xiaoai;Chen, Fang;Zhu, Yunxia;Li, Yating;Wu, Fu-Gen;Zhang, Yaqin;Lei, Ting;Han, Xiao
香菇多糖（LNT）是一种复杂的真菌成分，具有有效的抗肿瘤和免疫刺激特性。然而，关于LNT对1型糖尿病的作用和机制的研究还很少。在当前的研究中，我们研究了腹腔注射 LNT 是否可以降低非肥胖糖尿病 (NOD) 小鼠患 1 型糖尿病 (T1D) 的风险，并进一步研究了 LNT 作用的可能机制。方法：8周龄糖尿病前期雌性NOD小鼠，血糖水平140～160mg/dL、200～230mg/dL或350～450mg/dL的NOD小鼠随机分为两组，每隔一天腹腔注射5mg/kg LNT或PBS。然后，检查治疗小鼠的血糖水平、胰腺切片、脾脏、PnLN 和胰腺细胞。我们的结果表明，低剂量注射（5mg/kg）LNT 显着抑制患有自身免疫性糖尿病的小鼠的免疫病理学，但增加了小鼠中 Foxp3+ 调节性 T 细胞（Treg 细胞）的比例。 LNT 治疗在体外诱导 NOD 小鼠脾脏和 PnLN 细胞中产生 Tregs。此外，从 LNT 处理的 NOD 小鼠中提取的 Treg 细胞的过继转移证实，LNT 在体内诱导 Treg 功能，并且与从对照组分离的 Treg 相比，显示出增强的抑制能力。 LNT 能够刺激初始 CD4+T 细胞产生 Treg 细胞，这意味着 LNT 作为耐受性佐剂具有治疗价值，可用于逆转 T1D 早期和晚期的高血糖。
Lentinan (LNT) is a complex fungal component that possesses effective antitumor and immunostimulating properties. However, there is a paucity of studies regarding the effects and mechanisms of LNT on type 1 diabetes. In the current study, we investigated whether an intraperitoneal injection of LNT can diminish the risk of developing type 1 diabetes (T1D) in non-obese diabetic (NOD) mice and further examined possible mechanisms of LNT’s effects. Methods: Pre-diabetic female NOD mice 8 weeks of age, NOD mice with 140–160 mg/dL, 200–230 mg/dL or 350–450 mg/dL blood glucose levels were randomly divided into two groups and intraperitoneally injected with 5 mg/kg LNT or PBS every other day. Then, blood sugar levels, pancreas slices, spleen, PnLN and pancreas cells from treatment mice were examined. Our results demonstrated that low-dosage injections (5 mg/kg) of LNT significantly suppressed immunopathology in mice with autoimmune diabetes but increased the Foxp3+ regulatory T cells (Treg cells) proportion in mice. LNT treatment induced the production of Tregs in the spleen and PnLN cells of NOD mice in vitro. Furthermore, the adoptive transfer of Treg cells extracted from LNT-treated NOD mice confirmed that LNT induced Treg function in vivo and revealed an enhanced suppressive capacity as compared to the Tregs isolated from the control group. LNT was capable of stimulating the production of Treg cells from naive CD4 + T cells, which implies that LNT exhibits therapeutic values as a tolerogenic adjuvant and may be used to reverse hyperglycaemia in the early and late stages of T1D.
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影响因子：
17.1
作者：
Kasagi, Shimpei;Zhang, Pin;Chen, WanJun
通讯作者：
Chen, WanJun
DOI：
10.1097/mco.0b013e32833f1afb
发表时间：
2010-11-01
影响因子：
3.1
作者：
Murphy, E. Angela;Davis, J. Mark;Carmichael, Martin D.
通讯作者：
Carmichael, Martin D.
影响因子：
2.8
作者：
Ina, Kenji;Kataoka, Takae;Ando, Takafumi
通讯作者：
Ando, Takafumi
影响因子：
7.7
作者：
Rickels, Michael R.;Fuller, Carissa;Teff, Karen L.
通讯作者：
Teff, Karen L.
影响因子：
5.2
作者：
Chanput, Wasaporn;Reitsma, Marit;Wichers, Harry J.
通讯作者：
Wichers, Harry J.