A dynamic partitioning mechanism polarizes membrane protein distribution.
A dynamic partitioning mechanism polarizes membrane protein distribution.
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DOI：
10.1038/s41467-023-43615-2
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发表时间：
2023-11-30
影响因子：
16.6
通讯作者：
Iglesias, Pablo A.
中科院分区：
文献类型：
--
作者：
Banerjee, Tatsat;Matsuoka, Satomi;Biswas, Debojyoti;Miao, Yuchuan;Pal, Dhiman Sankar;Kamimura, Yoichiro;Ueda, Masahiro;Devreotes, Peter N.;Iglesias, Pablo A.
质膜被广泛认为是众多信号转导活动的中枢。然而，时空区隔不同类别膜蛋白的基本生物物理机制仍不清楚。利用多模态活细胞成像技术，研究人员首次发现Ras/PI3K/Akt/F-actin网络激活的膜区域中，几种脂质锚定膜蛋白持续减少。这些蛋白的动态极化不依赖于基于f -肌动蛋白的细胞骨架结构，膜和细胞质之间的反复穿梭，或定向的囊泡运输。光转换显微镜和单分子测量表明，这些脂质锚定分子在膜的不同区域具有本质上不同的扩散特征，这使得它们能够选择性分离。当这些扩散系数被纳入一个基于可激发网络的随机反应扩散模型时，模拟表明，改变的亲和介导的选择性分配足以驱动熟悉的传播波模式。此外，当膜结构域特异性肽被光遗传学招募到膜蛋白上时，通常均匀的整体和脂质锚定膜蛋白可以成功地分裂。我们提出“动态分配”作为一种新机制，可以解释在膜极化的各种生理过程中，广泛的脂质锚定和整体膜蛋白的大规模区隔化。不同的膜蛋白动态极化来组织信号转导，但其机制尚不清楚。在这里，作者表明，差异扩散介导的分配过程足以驱动膜相关信号蛋白的时空模式。
The plasma membrane is widely regarded as the hub of the numerous signal transduction activities. Yet, the fundamental biophysical mechanisms that spatiotemporally compartmentalize different classes of membrane proteins remain unclear. Using multimodal live-cell imaging, here we first show that several lipid-anchored membrane proteins are consistently depleted from the membrane regions where the Ras/PI3K/Akt/F-actin network is activated. The dynamic polarization of these proteins does not depend upon the F-actin-based cytoskeletal structures, recurring shuttling between membrane and cytosol, or directed vesicular trafficking. Photoconversion microscopy and single-molecule measurements demonstrate that these lipid-anchored molecules have substantially dissimilar diffusion profiles in different regions of the membrane which enable their selective segregation. When these diffusion coefficients are incorporated into an excitable network-based stochastic reaction-diffusion model, simulations reveal that the altered affinity mediated selective partitioning is sufficient to drive familiar propagating wave patterns. Furthermore, normally uniform integral and lipid-anchored membrane proteins partition successfully when membrane domain-specific peptides are optogenetically recruited to them. We propose “dynamic partitioning” as a new mechanism that can account for large-scale compartmentalization of a wide array of lipid-anchored and integral membrane proteins during various physiological processes where membrane polarizes. Different membrane proteins dynamically polarize to organize signal transduction, but the underlying mechanism is unclear. Here, the authors show that a differential diffusion mediated partitioning process is sufficient to drive such spatiotemporal patterning of membrane-associated signaling proteins.
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影响因子：
64.5
作者：
Freeman SA;Vega A;Riedl M;Collins RF;Ostrowski PP;Woods EC;Bertozzi CR;Tammi MI;Lidke DS;Johnson P;Mayor S;Jaqaman K;Grinstein S
通讯作者：
Grinstein S
由脂质力驱动的大量内吞作用，该脂质力源自外部浆膜单层：一种新的膜回收和脂质结构域的方法。
DOI：
10.1085/jgp.201010469
发表时间：
2011-02
期刊：
The Journal of general physiology
影响因子：
--
作者：
Fine M;Llaguno MC;Lariccia V;Lin MJ;Yaradanakul A;Hilgemann DW
通讯作者：
Hilgemann DW
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
13.6
作者：
Bhattacharya, Sayak;Banerjee, Tatsat;Iglesias, Pablo A.
通讯作者：
Iglesias, Pablo A.
DOI：
10.1146/annurev-cellbio-100616-060739
发表时间：
2017-10-06
影响因子：
11.3
作者：
Devreotes PN;Bhattacharya S;Edwards M;Iglesias PA;Lampert T;Miao Y
通讯作者：
Miao Y