Salmonella identified in pigs in Kenya and Malawi reveals the potential for zoonotic transmission in emerging pork markets.
Salmonella identified in pigs in Kenya and Malawi reveals the potential for zoonotic transmission in emerging pork markets.
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在肯尼亚和马拉维的猪中鉴定出的沙门氏菌揭示了新兴猪肉市场的人畜共患传播的潜力。
DOI：
10.1371/journal.pntd.0008796
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发表时间：
2020-11
影响因子：
3.8
通讯作者：
Fèvre EM
中科院分区：
文献类型：
--
作者：
Wilson CN;Pulford CV;Akoko J;Perez Sepulveda B;Predeus AV;Bevington J;Duncan P;Hall N;Wigley P;Feasey N;Pinchbeck G;Hinton JCD;Gordon MA;Fèvre EM
沙门氏菌是全球食源性疾病的主要原因。猪可以无症状地携带和散发非伤寒沙门氏菌（NTS），代表这些病原体的重要储存库。为了研究非洲家猪的沙门氏菌携带情况，于2016年10月至2017年5月期间在内罗毕、布西亚（肯尼亚）和奇夸瓦（马拉维）屠宰时采集了粪便和肠系膜淋巴结样本。对来自647头猪的样本进行了选择性培养、抗血清检测和全基因组测序;布西亚、内罗毕和Chikwawa的NTS携带率分别为12.7%、9.1%和24.6%。两株S.鼠伤寒ST 313被分离，但与英国胃肠炎相关的ST 313分离株的关系比非洲的血流感染更密切。在肯尼亚和马拉维发现的猪NTS携带揭示了在这些国家将猪病毒株传播给人类的潜在人畜共患传播，但不传播与非洲侵袭性NTS疾病特别相关的分支。感染非伤寒沙门氏菌（NTS）的健康人通常患有不经治疗即可消退的肠道疾病。然而，免疫系统受损的患者的NTS感染可导致血流感染或身体其他部位的感染;所谓的“侵入性”NTS感染。在过去的二十年里，NTS一直是撒哈拉以南非洲地区从人类血液中分离出的最普遍的细菌。众所周知，猪能够携带各种不同的NTS血清型，而自身不显示疾病迹象。携带者猪可能会间歇性地在粪便中排出NTS，这可能会导致人类疾病。如果在猪屠宰过程中没有遵守良好的卫生规程，供人食用的猪肉可能会被NTS污染。在未来几年，预计撒哈拉以南非洲的猪肉消费量将大幅增加。该地区猪无症状携带NTS的程度从未进行过详细调查。在这项研究中，我们报告说，在撒哈拉以南非洲的猪能够携带各种各样的非伤寒沙门氏菌血清型，如S。鼠伤寒，有可能导致人类的肠道疾病。没有发现以前与撒哈拉以南非洲侵袭性NTS疾病相关的NTS分支。
Salmonella is a major cause of foodborne disease globally. Pigs can carry and shed non-typhoidal Salmonella (NTS) asymptomatically, representing a significant reservoir for these pathogens. To investigate Salmonella carriage by African domestic pigs, faecal and mesenteric lymph node samples were taken at slaughter in Nairobi, Busia (Kenya) and Chikwawa (Malawi) between October 2016 and May 2017. Selective culture, antisera testing and whole genome sequencing were performed on samples from 647 pigs; the prevalence of NTS carriage was 12.7% in Busia, 9.1% in Nairobi and 24.6% in Chikwawa. Two isolates of S. Typhimurium ST313 were isolated, but were more closely related to ST313 isolates associated with gastroenteritis in the UK than bloodstream infection in Africa. The discovery of porcine NTS carriage in Kenya and Malawi reveals potential for zoonotic transmission of diarrhoeal strains to humans in these countries, but not for transmission of clades specifically associated with invasive NTS disease in Africa. Healthy humans infected with non-typhoidal Salmonella (NTS) typically suffer from diarrhoeal disease which resolves without treatment. However, NTS infection of patients with an impaired immune system can lead to either bloodstream infection or infection in another part of the body; so-called ‘invasive’ NTS infection. Over the last twenty years, NTS have been the most prevalent bacteria to be isolated from human blood in sub-Saharan Africa. It is well known that pigs are able to carry a wide range of different NTS serovars without showing signs of disease themselves. Carrier pigs may be able to intermittently shed NTS in their faeces which may have the potential to cause disease in humans. If good hygiene protocols are not followed during the porcine slaughter process, pork intended for human consumption may become contaminated with NTS. In the next few years, pork consumption is projected to increase dramatically in sub-Saharan Africa. The degree of asymptomatic carriage of NTS by pigs in this region has never been investigated in detail. In this study, we report that pigs in sub-Saharan Africa are able to carry a wide variety of non-typhoidal Salmonella serovars, such as S. Typhimurium, that have the potential to cause diarrhoeal disease in humans. No clades of NTS which have previously been associated with invasive NTS disease in sub-Saharan Africa were detected.
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DOI：
10.1093/bioinformatics/btp347
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Assefa S;Keane TM;Otto TD;Newbold C;Berriman M
通讯作者：
Berriman M
影响因子：
11.8
作者：
Feasey, Nicholas A.;Masesa, Clemens;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
影响因子：
30.8
作者：
Feasey, Nicholas A.;Hadfield, James;Keddy, Karen H.;Dallman, Timothy J.;Jacobs, Jan;Deng, Xiangyu;Wigley, Paul;Barquist, Lars Barquist;Langridge, Gemma C.;Feltwell, Theresa;Harris, Simon R.;Mather, Alison E.;Fookes, Maria;Aslett, Martin;Msefula, Chisomo;Kariuki, Samuel;Maclennan, Calman A.;Onsare, Robert S.;Weill, Francois-Xavier;Le Hello, Simon;Smith, Anthony M.;McClelland, Michael;Desai, Prerak;Parry, Christopher M.;Cheesbrough, John;French, Neil;Campos, Josefina;Chabalgoity, Jose A.;Betancor, Laura;Hopkins, Katie L.;Nair, Satheesh;Humphrey, Tom J.;Lunguya, Octavie;Cogan, Tristan A.;Tapia, Milagritos D.;Sow, Samba O.;Tennant, Sharon M.;Bornstein, Kristin;Levine, Myron M.;Lacharme-Lora, Lizeth;Everett, Dean B.;Kingsley, Robert A.;Parkhill, Julian;Heyderman, Robert S.;Dougan, Gordon;Gordon, Melita A.;Thomson, Nicholas R.
通讯作者：
Thomson, Nicholas R.
影响因子：
9.4
作者：
Larsen, Mette V.;Cosentino, Salvatore;Lund, Ole
通讯作者：
Lund, Ole
影响因子：
2.6
作者：
Baptista, F. M.;Dahl, J.;Nielsen, L. R.
通讯作者：
Nielsen, L. R.