Extracellular matrix of dental pulp stem cells: applications in pulp tissue engineering using somatic MSCs.
Extracellular matrix of dental pulp stem cells: applications in pulp tissue engineering using somatic MSCs.
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牙髓干细胞的细胞外基质：使用体细胞MSC在纸浆组织工程中的应用。
DOI：
10.3389/fphys.2013.00395
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发表时间：
2014-01-06
影响因子：
4
通讯作者：
George A
中科院分区：
文献类型：
--
作者：
Ravindran S;Huang CC;George A
龋齿影响着世界上大约90%的人口。目前临床上治疗龋齿的方法主要是根管治疗。这种治疗会导致牙齿失去敏感性和活力。组织工程可以通过使功能性牙髓组织再生来潜在地解决这个问题。牙髓干细胞（DPSC）已被证明是牙髓再生的良好来源。然而，这些细胞的有限可用性阻碍了其临床转化的潜力。我们已经研究了使用从其他来源的体间充质干细胞（MSC）的牙髓组织再生使用仿生牙髓细胞外基质（ECM）纳入支架的可能性。研究人牙周膜干细胞（PDLSCs）和人骨髓基质细胞（HMSCs）向牙源性谱系分化的能力。体外实时PCR结果结合组织学和免疫组织化学检查证实了PDLSC和HMSC形成血管化牙髓样组织的能力。这些发现表明，牙髓干细胞衍生的ECM支架刺激PDLSC和HMSC的牙向分化，而不需要外源性添加生长和分化因子。这项研究代表了一个翻译的角度对可能的治疗应用的组合使用体干细胞和细胞外基质的牙髓再生。
Dental Caries affects approximately 90% of the world's population. At present, the clinical treatment for dental caries is root canal therapy. This treatment results in loss of tooth sensitivity and vitality. Tissue engineering can potentially solve this problem by enabling regeneration of a functional pulp tissue. Dental pulp stem cells (DPSCs) have been shown to be an excellent source for pulp regeneration. However, limited availability of these cells hinders its potential for clinical translation. We have investigated the possibility of using somatic mesenchymal stem cells (MSCs) from other sources for dental pulp tissue regeneration using a biomimetic dental pulp extracellular matrix (ECM) incorporated scaffold. Human periodontal ligament stem cells (PDLSCs) and human bone marrow stromal cells (HMSCs) were investigated for their ability to differentiate toward an odontogenic lineage. In vitro real-time PCR results coupled with histological and immunohistochemical examination of the explanted tissues confirmed the ability of PDLSCs and HMSCs to form a vascularized pulp-like tissue. These findings indicate that the dental pulp stem derived ECM scaffold stimulated odontogenic differentiation of PDLSCs and HMSCs without the need for exogenous addition of growth and differentiation factors. This study represents a translational perspective toward possible therapeutic application of using a combination of somatic stem cells and extracellular matrix for pulp regeneration.
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影响因子：
--
作者：
GOULD, TRL;MELCHER, AH;BRUNETTE, DM
通讯作者：
BRUNETTE, DM
影响因子：
4.2
作者：
Wang, Yuanyuan;Zhao, Yuming;Ge, Lihong
通讯作者：
Ge, Lihong
影响因子：
3.7
作者：
Sonoyama, Wataru;Liu, Yi;Fang, Dianji;Yamaza, Takayoshi;Seo, Byoung-Moo;Zhang, Chunmei;Liu, He;Gronthos, Stan;Wang, Cun-Yu;Shi, Songtao;Wang, Songlin
通讯作者：
Wang, Songlin
影响因子：
4.1
作者：
Rowe, PSN;Garrett, IR;Gutierrez, GE
通讯作者：
Gutierrez, GE
影响因子：
4.1
作者：
Ravindran, Sriram;Song, Yiqiang;George, Anne
通讯作者：
George, Anne