Rice root-associated bacteria: insights into community structures across 10 cultivars.
Rice root-associated bacteria: insights into community structures across 10 cultivars.
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DOI：
10.1111/j.1574-6941.2011.01092.x
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发表时间：
2011-07
影响因子：
4.2
通讯作者：
van Elsas JD
中科院分区：
文献类型：
--
作者：
Hardoim PR;Andreote FD;Reinhold-Hurek B;Sessitsch A;van Overbeek LS;van Elsas JD
在这项研究中，调查了植物基因型、土壤类型和养分利用效率对与水稻根系相关的不同细菌群落组成的影响。因此，利用聚合酶链式反应（PCR）结合变性梯度凝胶电泳（PCR - DGGE）对总细菌、α - 变形菌和β - 变形菌、假单胞菌和放线菌进行了研究。水稻基因型在很大程度上决定了不同品种间不同细菌群落的组成。几个属于籼稻亚种的品种倾向于选择相似的细菌群落，而属于粳稻和香稻亚种的品种则选择群落结构不同的细菌群落。土壤类型对放线菌群落有显著影响，而对于所分析的所有群落，“改良”和“传统”植株的影响较小。PCR - DGGE中的一些优势条带，与放射形土壤杆菌、玉米迪基氏菌、博莱特分枝杆菌以及根瘤菌目、红螺菌科和类芽孢杆菌科的成员相关，分布在不同品种中。相比之下，大多数条带（例如与阴沟肠杆菌或库氏伯克霍尔德氏菌相关的条带）仅存在于特定品种中，或者在水稻重复样本中不规则分布。数据表明，细菌的适应性和植物基因型都对与水稻植株根系相关的动态细菌群落的形成有贡献。
In this study, the effect of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated. Thus, total bacteria, Alpha- and Beta-proteobacteria, Pseudomonas and Actinobacteria were studied using PCR followed by denaturing gradient gel electrophoresis (PCR-DGGE). Rice genotype determined to a large extent the composition of the different bacterial communities across cultivars. Several cultivars belonging to Oryza sativa subspecies indica tended to select similar bacterial communities, whereas those belonging to subspecies japonica and aromatica selected ones with divergent community structures. An effect of soil type was pronounced for the Actinobacteria communities, while a small effect of ‘improved’ and ‘traditional’ plants was noted for all communities analysed. A few dominant bands in PCR-DGGE, affiliated with Rhizobium radiobacter, Dickeya zeae, Mycobacterium bolletii and with members of the Rhizobiales, Rhodospirillaceae and Paenibacillaceae were spread across cultivars. In contrast, a majority of bands (e.g. affiliated with Enterobacter cloacae or Burkholderia kururiensis) was only present in particular cultivars or was erratically distributed amongst rice replicates. The data suggested that both bacterial adaptation and plant genotype contribute to the shaping of the dynamic bacterial communities associated with roots of rice plants.
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影响因子：
4.2
作者：
Costa, R;Götz, M;Smalla, K
通讯作者：
Smalla, K
影响因子：
2.7
作者：
Dreo, T.;Gruden, K.;Ravnikar, M.
通讯作者：
Ravnikar, M.
影响因子：
4.1
作者：
Hurek, T;Reinhold-Hurek, B
通讯作者：
Reinhold-Hurek, B
影响因子：
3.6
作者：
Chelius, MK;Triplett, EW
通讯作者：
Triplett, EW
影响因子：
5.1
作者：
Engelhard, M;Hurek, T;Reinhold-Hurek, B
通讯作者：
Reinhold-Hurek, B